ArticlePloS one2010
Epimorphin regulates bile duct formation via effects on mitosis orientation in rat liver epithelial stem-like cells.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed, 16 citations in OpenAlex.
- Elasticity and tumorigenic characteristics of cells in a monolayer after nanosecond pulsed electric field exposure.European biophysics journal : EBJ · 2017Article
- Article
- Epimorphin alters the inhibitory effects of SOX9 on Mmp13 in activated hepatic stellate cells.PloS one · 2014Article
- Stages based molecular mechanisms for generating cholangiocytes from liver stem/progenitor cells.World journal of gastroenterology · 2013Review
- A polymorphism in the integrin αV subunit gene affects the progression of primary biliary cirrhosis in Japanese patients.Journal of gastroenterology · 2011Article
- Alveolar type II cells escape stress failure caused by tonic stretch through transient focal adhesion disassembly.International journal of biological sciences · 2011Article
Corrections and comments
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Authors and funding
15 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding how hepatic precursor cells can generate differentiated bile ducts is crucial for studies on epithelial morphogenesis and for development of cell therapies for hepatobiliary diseases. Epimorphin (EPM) is a key morphogen for duct morphogenesis in various epithelial organs. The role of EPM in bile duct formation (DF) from hepatic precursor cells, however, is not known. To address this issue, we used WB-F344 rat epithelial stem-like cells as model for bile duct formation. A micropattern and a uniaxial static stretch device was used to investigate the effects of EPM and stress fiber bundles on the mitosis orientation (MO) of WB cells. Immunohistochemistry of liver tissue sections demonstrated high EPM expression around bile ducts in vivo. In vitro, recombinant EPM selectively induced DF through upregulation of CK19 expression and suppression of HNF3alpha and HNF6, with no effects on other hepatocytic genes investigated. Our data provide evidence that EPM guides MO of WB-F344 cells via effects on stress fiber bundles and focal adhesion assembly, as supported by blockade EPM, beta1 integrin, and F-actin assembly. These blockers can also inhibit EPM-induced DF. These results demonstrate a new biophysical action of EPM in bile duct formation, during which determination of MO plays a crucial role.
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Registered trials
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