ArticleEukaryotic cell2010
Homoserine toxicity in Saccharomyces cerevisiae and Candida albicans homoserine kinase (thr1Delta) mutants.
Article in Eukaryotic cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 42 citations in OpenAlex.
- Beyond Protein Building Blocks: A Review of Biological Roles and Therapeutic Potential of Free Amino Acids.International journal of molecular sciences · 2025Review
- The interactome of the Bakers' yeast peroxiredoxin Tsa1 implicates it in redox regulation of intermediary metabolism, glycolysis, and zinc homeostasis.Metallomics : integrated biometal science · 2025Article
- A novel plasmid-based experimental system in Saccharomyces cerevisiae that enables the introduction of 10 different plasmids into cells.FEBS open bio · 2024Article
- A highly conserved tRNA modification contributes toMicrobiology spectrum · 2024Article
- Metabolic Engineering ofChem & bio engineering · 2024Review
- Lethal metabolism of Candida albicans respiratory mutants.PloS one · 2024Article
- Evaluation of Cell Responses ofMicroorganisms · 2023Article
- Toxic effect and inability of L-homoserine to be a nitrogen source for growth ofFrontiers in microbiology · 2022Article
- Molecular targets for antifungals in amino acid and protein biosynthetic pathways.Amino acids · 2021Review
- More Than Just Cleaning: Ubiquitin-Mediated Proteolysis in Fungal Pathogenesis.Frontiers in cellular and infection microbiology · 2021Review
- Rational Proteomic Analysis of a New DomesticatedFrontiers in microbiology · 2021Article
- THR1 mediates GCN4 and CDC4 to link morphogenesis with nutrient sensing and the stress response in Candida albicans.International journal of molecular medicine · 2018Article
- Potential targets for the development of new antifungal drugs.The Journal of antibiotics · 2018Review
- Article
- Identification of Genes inG3 (Bethesda, Md.) · 2017Article
- Optimality principles reveal a complex interplay of intermediate toxicity and kinetic efficiency in the regulation of prokaryotic metabolism.PLoS computational biology · 2017Article
- Metabolic engineering of Escherichia coli W3110 for the production of L-methionine.Journal of industrial microbiology & biotechnology · 2017Article
- Improving the flux distributions simulated with genome-scale metabolic models ofMetabolic engineering communications · 2016Article
- Global translational impacts of the loss of the tRNA modification tMicrobial cell (Graz, Austria) · 2016Article
- Homologous trans-editing factors with broad tRNA specificity prevent mistranslation caused by serine/threonine misactivation.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
In addition to threonine auxotrophy, mutation of the Saccharomyces cerevisiae threonine biosynthetic genes THR1 (encoding homoserine kinase) and THR4 (encoding threonine synthase) results in a plethora of other phenotypes. We investigated the basis for these other phenotypes and found that they are dependent on the toxic biosynthetic intermediate homoserine. Moreover, homoserine is also toxic for Candida albicans thr1Delta mutants. Since increasing levels of threonine, but not other amino acids, overcome the homoserine toxicity of thr1Delta mutants, homoserine may act as a toxic threonine analog. Homoserine-mediated lethality of thr1Delta mutants is blocked by cycloheximide, consistent with a role for protein synthesis in this lethality. We identified various proteasome and ubiquitin pathway components that either when mutated or present in high copy numbers suppressed the thr1Delta mutant homoserine toxicity. Since the doa4Delta and proteasome mutants identified have reduced ubiquitin- and/or proteasome-mediated proteolysis, the degradation of a particular protein or subset of proteins likely contributes to homoserine toxicity.
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Registered trials
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