ArticleEukaryotic cell2010
A Plasmodium falciparum transcriptional cyclin-dependent kinase-related kinase with a crucial role in parasite proliferation associates with histone deacetylase activity.
Article in Eukaryotic cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 47 citations in OpenAlex.
- Arrest of nuclear division in Plasmodium through blockage of erythrocyte surface exposed ribosomal protein P2.PLoS pathogens · 2012Trial
- Integrative genomics ofbioRxiv : the preprint server for biology · 2026Article
- Targeting Malaria's Achilles' Heels: A Review of Plasmodium Life Cycle Vulnerabilities for Drug Discovery.Current topics in medicinal chemistry · 2025Review
- PfHDAC1 is an essential regulator ofmBio · 2024Article
- An Update on Development of Small-Molecule Plasmodial Kinase Inhibitors.Molecules (Basel, Switzerland) · 2020Review
- Overexpression of the HECT ubiquitin ligase PfUT prolongs the intraerythrocytic cycle and reduces invasion efficiency of Plasmodium falciparum.Scientific reports · 2019Article
- Checks and balances? DNA replication and the cell cycle in Plasmodium.Parasites & vectors · 2018Review
- Checkpoints of apicomplexan cell division identified in Toxoplasma gondii.PLoS pathogens · 2017Article
- Dynamic and Combinatorial Landscape of Histone Modifications during the Intraerythrocytic Developmental Cycle of the Malaria Parasite.Journal of proteome research · 2016Article
- Regulation of DNA replication proteins in parasitic protozoans: possible role of CDK-like kinases.Current genetics · 2016Review
- Correlation between Cyclin Dependent Kinases and Artemisinin-Induced Dormancy in Plasmodium falciparum In Vitro.PloS one · 2016Article
- Chemical interrogation of the malaria kinome.Chembiochem : a European journal of chemical biology · 2014Article
- Cell division in apicomplexan parasites.Nature reviews. Microbiology · 2014Review
- Mefloquine exposure induces cell cycle delay and reveals stage-specific expression of the pfmdr1 gene.Antimicrobial agents and chemotherapy · 2013Article
- An evolutionary perspective on the kinome of malaria parasites.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2012Review
- Toxoplasma and Plasmodium protein kinases: roles in invasion and host cell remodelling.International journal for parasitology · 2012Review
- Global kinomic and phospho-proteomic analyses of the human malaria parasite Plasmodium falciparum.Nature communications · 2011Article
- Plasmodium falciparum NIMA-related kinase Pfnek-1: sex specificity and assessment of essentiality for the erythrocytic asexual cycle.Microbiology (Reading, England) · 2011Article
- The Cryptosporidium parvum kinome.BMC genomics · 2011Article
- Artemisinin resistance in Plasmodium falciparum is associated with an altered temporal pattern of transcription.BMC genomics · 2011Article
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Authors and funding
10 authors at 5 institutions in 4 countries.
Funding
Abstract
Cyclin-dependent protein kinases (CDKs) are key regulators of the eukaryotic cell cycle and of the eukaryotic transcription machinery. Here we report the characterization of Pfcrk-3 (Plasmodium falciparum CDK-related kinase 3; PlasmoDB identifier PFD0740w), an unusually large CDK-related protein whose kinase domain displays maximal homology to those CDKs which, in other eukaryotes, are involved in the control of transcription. The closest enzyme in Saccharomyces cerevisiae is BUR1 (bypass upstream activating sequence requirement 1), known to control gene expression through interaction with chromatin modification enzymes. Consistent with this, immunofluorescence data show that Pfcrk-3 colocalizes with histones. We show that recombinant Pfcrk-3 associates with histone H1 kinase activity in parasite extracts and that this association is detectable even if the catalytic domain of Pfcrk-3 is rendered inactive by site-directed mutagenesis, indicating that Pfcrk-3 is part of a complex that includes other protein kinases. Immunoprecipitates obtained from extracts of transgenic parasites expressing hemagglutinin (HA)-tagged Pfcrk-3 by using an anti-HA antibody displayed both protein kinase and histone deacetylase activities. Reverse genetics data show that the pfcrk-3 locus can be targeted only if the genetic modification does not cause a loss of function. Taken together, our data strongly suggest that Pfcrk-3 fulfils a crucial role in the intraerythrocytic development of P. falciparum, presumably through chromatin modification-dependent regulation of gene expression.
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