ArticlePlant physiology2010
Functional cyanobacterial beta-carboxysomes have an absolute requirement for both long and short forms of the CcmM protein.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 110 citations in OpenAlex.
- Advances in Decoding Bacterial N-Terminal Proteoforms: Technologies, Challenges, and Functional Insights.Microorganisms · 2026Review
- From fundamental understanding to engineering of carboxysomes for biotechnological applications.Plant communications · 2026Review
- Overexpression of β-carboxysomes increases photosynthesis and growth in Synechocystis sp. PCC 6803.Plant physiology · 2026Article
- Functional and structural insights into cyanobacterial COThe Plant journal : for cell and molecular biology · 2026Review
- Stages of biomolecular condensate formation in pro-β-carboxysome assembly.Nature plants · 2026Article
- Understanding carboxysomes to enhance carbon fixation in crops.Biochemical Society transactions · 2025Review
- Engineering COPlant communications · 2025Article
- Rubisco packaging and stoichiometric composition of the native β-carboxysome in Synechococcus elongatus PCC7942.Plant physiology · 2024Article
- Molecular interactions of the chaperone CcmS and carboxysome shell protein CcmK1 that mediate β-carboxysome assembly.Plant physiology · 2024Article
- Rubisco packaging and stoichiometric composition of a native β-carboxysome.bioRxiv : the preprint server for biology · 2024Article
- Towards engineering a hybrid carboxysome.Photosynthesis research · 2023Article
- Adapting from Low to High: An Update to COPlants (Basel, Switzerland) · 2023Review
- Microcompartment assembly around multicomponent fluid cargoes.The Journal of chemical physics · 2022Article
- Decoding the Absolute Stoichiometric Composition and Structural Plasticity of α-Carboxysomes.mBio · 2022Article
- Scaffolding protein CcmM directs multiprotein phase separation in β-carboxysome biogenesis.Nature structural & molecular biology · 2021Article
- Complex structure reveals CcmM and CcmN form a heterotrimeric adaptor in β-carboxysome.Protein science : a publication of the Protein Society · 2021Article
- New discoveries expand possibilities for carboxysome engineering.Current opinion in microbiology · 2021Review
- Review
- Article
- Rubisco accumulation factor 1 (Raf1) plays essential roles in mediating Rubisco assembly and carboxysome biogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carboxysomes are an essential part of the cyanobacterial CO2-concentrating mechanism, consisting of a protein shell and an interior of Rubisco. The beta-carboxysome shell protein CcmM forms two peptides via a proposed internal ribosomal entry site (IRES) within the ccmM transcript in Synechococcus PCC7942. The abundant short form (35 kD, M35) consists of Rubisco small subunit-like repeats and binds Rubisco. The lower abundance long form (58 kD, M58) also contains a gamma-carbonic anhydrase-like domain, which binds the carboxysomal carbonic anhydrase, CcaA. We examined whether these CcmM forms arise via an IRES or by other means. Mutations of a putative internal start codon (GTG) and Shine-Dalgarno sequence within ccmM, along with a gene coding for M35 alone, were examined in the high-CO2-requiring (HCR) carboxysomeless mutant, DeltaccmM. Expression of wild-type ccmM in DeltaccmM restored the wild-type phenotype, while mutation of putative start and Shine-Dalgarno sequences led to as much as 20-fold reduction in M35 content with no recovery from HCR phenotype. These cells also contained small electron-dense structures. Cells producing little or no M58, but sufficient M35, were found to contain large electron-dense structures, no CcaA, and had a HCR phenotype. Large subcellular aggregates can therefore form in the absence of M58, suggesting a role for M35 in internal carboxysome Rubisco packing. The results confirm that M35 is independently translated via an IRES within ccmM. Importantly, the data reveal that functional carboxysomes require both M35 and M58 in sufficient quantities and with a minimum stoichiometry of close to 1:1.
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