ArticleThe Journal of biological chemistry2010
Hidden function of catalytic domain in 6-methylsalicylic acid synthase for product release.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 46 citations in OpenAlex.
- A hybrid assembly mechanism employs nonreducing polyketide synthase-like logic for partially reduced polyketide formation.Marine life science & technology · 2026Article
- The enzyme kinetics of branched-chain fatty acid synthesis of metazoan fatty acid synthase.Protein science : a publication of the Protein Society · 2025Article
- The Kinetics of Carbon-Carbon Bond Formation in Metazoan Fatty Acid Synthase and Its Impact on Product Fidelity.Angewandte Chemie (International ed. in English) · 2025Article
- Analyzing and engineering of the biosynthetic pathway of mollemycin A for enhancing its production.Synthetic and systems biotechnology · 2024Article
- Enzymology of assembly line synthesis by modular polyketide synthases.Nature chemical biology · 2023Review
- Strategies in megasynthase engineering - fatty acid synthases (FAS) as model proteins.Beilstein journal of organic chemistry · 2017Review
- Insights into 6-Methylsalicylic Acid Bio-assembly by Using Chemical Probes.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2016Article
- Insights into 6-Methylsalicylic Acid Bio-assembly by Using Chemical Probes.Angewandte Chemie (International ed. in English) · 2016Article
- An in planta-expressed polyketide synthase produces (R)-mellein in the wheat pathogen Parastagonospora nodorum.Applied and environmental microbiology · 2015Article
- Molecular genetic characterization of terreic acid pathway in Aspergillus terreus.Organic letters · 2014Article
- Sulfonyl 3-alkynyl pantetheinamides as mechanism-based cross-linkers of acyl carrier protein dehydratase.Journal of the American Chemical Society · 2013Article
- Active site comparisons and catalytic mechanisms of the hot dog superfamily.Chemical reviews · 2013Review
- Construction and engineering of large biochemical pathways via DNA assembler.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Navigating the fungal polyketide chemical space: from genes to molecules.The Journal of organic chemistry · 2012Article
- Dehydratase-specific probes for fatty acid and polyketide synthases.Journal of the American Chemical Society · 2012Article
- DNA assembler: a synthetic biology tool for characterizing and engineering natural product gene clusters.Methods in enzymology · 2012Article
- Rapid characterization and engineering of natural product biosynthetic pathways via DNA assembler.Molecular bioSystems · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Functional investigation of the proposed dehydratase domain of ATX, a 6-methylsalicylic acid synthase from Aspergillus terreus, revealed that the domain is not involved in dehydration of the beta-hydroxytriketide intermediate tethered on the acyl carrier protein but catalyzes thioester hydrolysis to release the product from the acyl carrier protein. Thus, we renamed this domain the thioester hydrolase (TH) domain. The intermediate bound to the TH domain of mutant H972A formed in the presence of NADPH was released as 6-methylsalicylic acid by both the intact ATX and by THID (a 541-amino acid region containing TH domain and its downstream) protein, in trans. Furthermore, THID showed a catalytic activity to hydrolyze a model substrate, 6-methylsalicylic acid-N-acetylcysteamine. The TH domain is the first example of a product-releasing domain that is located in the middle of a multidomain iterative type I polyketide synthase. Moreover, it is functionally different from serine protease-type thioesterase domains of iterative type I polyketide synthases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.