ArticleBiomaterials2010
Synergistic effects of tethered growth factors and adhesion ligands on DNA synthesis and function of primary hepatocytes cultured on soft synthetic hydrogels.
Article in Biomaterials, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 48 citations in OpenAlex.
- Molecular Engineering of Recombinant Protein Hydrogels: Programmable Design and Biomedical Applications.Gels (Basel, Switzerland) · 2025Review
- Directing Cholangiocyte Morphogenesis in Natural Biomaterial Scaffolds.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Oncoimmunology Meets Organs-on-Chip.Frontiers in molecular biosciences · 2021Review
- A polyelectrolyte multilayer platform for investigating growth factor delivery modes in human liver cultures.Journal of biomedical materials research. Part A · 2018Article
- On-demand dissolution of modular, synthetic extracellular matrix reveals local epithelial-stromal communication networks.Biomaterials · 2017Article
- Immobilized epidermal growth factor stimulates persistent, directed keratinocyte migration via activation of PLCγ1.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
- Degradable hydrogels derived from PEG-diacrylamide for hepatic tissue engineering.Journal of biomedical materials research. Part A · 2015Article
- Covalent Modification of Synthetic Hydrogels with Bioactive Proteins via Sortase-Mediated Ligation.Biomacromolecules · 2015Article
- Photopatterning of hydrogel scaffolds coupled to filter materials using stereolithography for perfused 3D culture of hepatocytes.Biotechnology and bioengineering · 2015Article
- Substrate stiffness regulates primary hepatocyte functions.RSC advances · 2015Article
- A composite hydrogel platform for the dissection of tumor cell migration at tissue interfaces.Biomaterials · 2014Article
- Engineering liver.Hepatology (Baltimore, Md.) · 2014Review
- Bioreactor technologies to support liver function in vitro.Advanced drug delivery reviews · 2014Review
- HelixSoft matter · 2012Article
- A material's point of view on recent developments of polymeric biomaterials: control of mechanical and biochemical properties.Journal of materials chemistry · 2011Article
- Article
- Autocrine-controlled formation and function of tissue-like aggregates by primary hepatocytes in micropatterned hydrogel arrays.Tissue engineering. Part A · 2011Article
- Engineering a stem cell house into a home.Stem cell research & therapy · 2011Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The composition, presentation, and spatial orientation of extracellular matrix molecules and growth factors are key regulators of cell behavior. Here, we used self-assembling peptide nanofiber gels as a modular scaffold to investigate how fibronectin-derived adhesion ligands and different modes of epidermal growth factor (EGF) presentation synergistically regulate multiple facets of primary rat hepatocyte behavior in the context of a soft gel. In the presence of soluble EGF, inclusion of dimeric RGD and the heparin binding domain from fibronectin (HB) increased hepatocyte aggregation, spreading, and metabolic function compared to unmodified gels or gels modified with a single motif, but unlike rigid substrates, gels failed to induce DNA synthesis. Tethered EGF dramatically stimulated cell aggregation and spreading under all adhesive ligand conditions and also preserved metabolic function. Surprisingly, tethered EGF elicited DNA synthesis on gels with RGD and HB. Phenotypic differences between soluble and tethered EGF stimulation of cells on peptide gels are correlated with differences in expression and phosphorylation the EGF receptor and its heterodimerization partner ErbB2, and activation of the downstream signaling node ERK1/2. These modular matrices reveal new facets of hepatocellular biology in culture and may be more broadly useful in culture of other soft tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.