ArticleJournal of virological methods2010
Simultaneous typing and HA/NA subtyping of influenza A and B viruses including the pandemic influenza A/H1N1 2009 by multiplex real-time RT-PCR.
Article in Journal of virological methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- UMPlex™: a targeted next-generation sequencing primer design workflow.Virology journal · 2025Article
- Epidemiological and molecular characteristics of human parainfluenza virus in southern China during 2016-2020.Virologica Sinica · 2025Article
- The sampling efficiencies of commercial nasopharyngeal swabs.Biosafety and health · 2022Article
- Influence of primer & probe chemistry and amplification target on reverse transcription digital PCR quantification of viral RNA.Biomolecular detection and quantification · 2016Article
- Diagnostics and surveillance for Swine influenza.Current topics in microbiology and immunology · 2013Review
- Subtyping influenza A virus with monoclonal antibodies and an indirect immunofluorescence assay.Journal of clinical microbiology · 2012Article
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- Validation and diagnostic application of NS and HA gene-specific real-time reverse transcription-PCR assays for detection of 2009 pandemic influenza A (H1N1) viruses in clinical specimens.Journal of clinical microbiology · 2011Article
- Evaluation of twelve real-time reverse transcriptase PCR primer-probe sets for detection of pandemic influenza A/H1N1 2009 virus.Journal of clinical microbiology · 2011Article
- Evaluation of a rapid diagnostic test, NanoSign® Influenza A/B Antigen, for detection of the 2009 pandemic influenza A/H1N1 viruses.Virology journal · 2010Article
- The role of rapid antigen testing for influenza in the era of molecular diagnostics.Molecular diagnosis & therapy · 2010Review
Corrections and comments
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8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pandemic influenza A/H1N1 2009 and seasonal influenza viruses are currently co-circulating worldwide. A rapid, sensitive, and specific assay for distinguishing pandemic influenza A/H1N1 2009 from seasonal influenza viruses and for subtyping seasonal influenza A viruses could aid in the surveillance and control of these viral infections. Here, such a multiplex real-time RT-PCR (rRT-PCR) assay for typing influenza A and B viruses and the pandemic influenza A/H1N1 2009 is developed. This assay can also subtype seasonal influenza A viruses simultaneously. The analytical sensitivity is 10-10(4) copies/reaction. The coefficients of variation of inter-assay and intra-assay are 0.04-0.45% and 0.08-0.97%, respectively. The new multiplex rRT-PCR assay is more sensitive in subtyping seasonal influenza viruses than the conventional PCR techniques. Results obtained with this assay for the detection of pandemic influenza A/H1N1 2009 are highly consistent (96.88%) with those achieved using the US CDC's rRT-PCR protocol. A sample identified as "pandemic influenza A/H1N1 2009 positive" by the US CDC's rRT-PCR was reclassified correctly as subtype H3N2 using this assay. Taken together, this new multiplex rRT-PCR protocol could be an important tool for improving diagnosis and management of the pandemic influenza A/H1N1 2009 and seasonal influenza viruses.
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