ArticleBiophysical journal2010
Force generation in lamellipodia is a probabilistic process with fast growth and retraction events.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Mechanotransduction as a major driver of cell behaviour: mechanisms, and relevance to cell organization and future research.Open biology · 2021Review
- An Integrated Cytoskeletal Model of Neurite Outgrowth.Frontiers in cellular neuroscience · 2018Review
- Cell-cell adhesion interface: orthogonal and parallel forces from contraction, protrusion, and retraction.F1000Research · 2018Review
- Local Arp2/3-dependent actin assembly modulates applied traction force during apCAM adhesion site maturation.Molecular biology of the cell · 2017Article
- Article
- Substrate Deformation Predicts Neuronal Growth Cone Advance.Biophysical journal · 2015Article
- HDAC6 and RhoA are novel players in Abeta-driven disruption of neuronal polarity.Nature communications · 2015Article
- The role of myosin-II in force generation of DRG filopodia and lamellipodia.Scientific reports · 2015Article
- Quantifying mechanical force in axonal growth and guidance.Frontiers in cellular neuroscience · 2015Review
- Maturation of filopodia shaft adhesions is upregulated by local cycles of lamellipodia advancements and retractions.PloS one · 2014Article
- Article
- The role of membrane stiffness and actin turnover on the force exerted by DRG lamellipodia.Biophysical journal · 2012Article
- Membrane dynamics shape TCR-generated signaling.Frontiers in immunology · 2012Article
- Real-time electrical measurement of L929 cellular spontaneous and synchronous oscillation.International journal of nanomedicine · 2012Article
- Cytoskeletal actin networks in motile cells are critically self-organized systems synchronized by mechanical interactions.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The formation of actin waves during regeneration after axonal lesion is enhanced by BDNF.Scientific reports · 2011Article
- The elementary events underlying force generation in neuronal lamellipodia.Scientific reports · 2011Article
- Stretchy proteins on stretchy substrates: the important elements of integrin-mediated rigidity sensing.Developmental cell · 2010Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polymerization of actin filaments is the primary source of motility in lamellipodia and it is controlled by a variety of regulatory proteins. The underlying molecular mechanisms are only partially understood and a precise determination of dynamical properties of force generation is necessary. Using optical tweezers, we have measured with millisecond (ms) temporal resolution and picoNewton (pN) sensitivity the force-velocity (Fv) relationship and the power dissipated by lamellipodia of dorsal root ganglia neurons. When force and velocity are averaged over 3-5 s, the Fv relationships can be flat. On a finer timescale, random occurrence of fast growth and subsecond retractions become predominant. The maximal power dissipated by lamellipodia over a silica bead with a diameter of 1 microm is 10(-16) W. Our results clarify the dynamical properties of force generation: i), force generation is a probabilistic process; ii), underlying biological events have a bandwidth up to at least 10 Hz; and iii), fast growth of lamellipodia leading edge alternates with local retractions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.