ArticleExperimental cell research2010
Heparin II domain of fibronectin mediates contractility through an alpha4beta1 co-signaling pathway.
Article in Experimental cell research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 25 citations in OpenAlex.
- Integrin α4 and α9 in trabecular meshwork and Schlemm's canal function: emerging regulators of intraocular pressure homeostasis.Frontiers in cell and developmental biology · 2026Review
- Rho Kinase Inhibitor AR-12286 Reverses Steroid-Induced Changes in Intraocular Pressure, Effective Filtration Areas, and Morphology in Mouse Eyes.Investigative ophthalmology & visual science · 2023Article
- Integrin Crosstalk and Its Effect on the Biological Functions of the Trabecular Meshwork/Schlemm's Canal.Frontiers in cell and developmental biology · 2022Review
- The Effects of Mechanical Stretch on Integrins and Filopodial-Associated Proteins in Normal and Glaucomatous Trabecular Meshwork Cells.Frontiers in cell and developmental biology · 2022Article
- Review
- Effect of αvβ3 Integrin Expression and Activity on Intraocular Pressure.Investigative ophthalmology & visual science · 2019Article
- The role of integrins in glaucoma.Experimental eye research · 2017Review
- Netarsudil Increases Outflow Facility in Human Eyes Through Multiple Mechanisms.Investigative ophthalmology & visual science · 2016Article
- Tissue-based multiphoton analysis of actomyosin and structural responses in human trabecular meshwork.Scientific reports · 2016Article
- Recent Developments in Understanding the Role of Aqueous Humor Outflow in Normal and Primary Open Angle Glaucoma.Current ophthalmology reports · 2015Article
- Aqueous humor outflow: dynamics and disease.Investigative ophthalmology & visual science · 2015Review
- Viral Vector Effects on Exoenzyme C3 Transferase-Mediated Actin Disruption and on Outflow Facility.Investigative ophthalmology & visual science · 2015Article
- Aqueous outflow: segmental and distal flow.Journal of cataract and refractive surgery · 2014Review
- Vascular biomechanical properties in mice with smooth muscle specific deletion of Ndst1.Molecular and cellular biochemistry · 2014Article
- Cellular fibronectin expression in human trabecular meshwork and induction by transforming growth factor-β2.Investigative ophthalmology & visual science · 2013Article
- Activated αvβ3 integrin regulates αvβ5 integrin-mediated phagocytosis in trabecular meshwork cells.Investigative ophthalmology & visual science · 2013Article
- Integrins in trabecular meshwork and optic nerve head: possible association with the pathogenesis of glaucoma.BioMed research international · 2013Review
- Two-photon immunofluorescence characterization of the trabecular meshwork in situ.Investigative ophthalmology & visual science · 2012Article
- Current understanding of conventional outflow dysfunction in glaucoma.Current opinion in ophthalmology · 2012Review
- Vascular cell adhesion molecule-1 expression and signaling during disease: regulation by reactive oxygen species and antioxidants.Antioxidants & redox signaling · 2011Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
In the trabecular meshwork (TM) of the eye, regulation of tissue contractility by the PPRARI sequence within the Heparin II (HepII) domain of fibronectin is believed to control the movement of aqueous humor and dictate the level of intraocular pressure. This study shows that the HepII domain utilizes activated alpha4beta1 integrin and collagen to mediate a co-signaling pathway that down-regulates contractility in TM cells. siRNA silencing of alpha4beta1 integrin blocked the actin disrupting effects of both PPRARI and the HepII domain. The down-regulation of the actin cytoskeleton and contractility did not involve syndecan-4 or other heparan sulfate proteoglycans (HSPGs) since siRNA silencing of syndecan-4 expression or heparitinase removal of cell surface HSPGs did not prevent the HepII-mediated disruption of the actin cytoskeleton. HepII-mediated disruption of the cytoskeleton depended upon the presence of collagen in the extracellular matrix, and cell binding studies indicated that HepII signaling involved cross-talk between alpha4beta1 and alpha1/alpha2beta1 integrins. This is the first time that the PPRARI sequence in the HepII domain has been shown to serve as a physiological alpha4beta1 ligand, suggesting that alpha4beta1 integrin may be a key regulator of tissue contractility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.