ArticleChembiochem : a European journal of chemical biology2010
RNA-cleaving deoxyribozyme sensor for nucleic acid analysis: the limit of detection.
Article in Chembiochem : a European journal of chemical biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- Multicomponent DNA Nanomachines for Amplification-Free Viral RNA Detection.International journal of molecular sciences · 2025Article
- Interrogation of highly structured RNA with multicomponent deoxyribozyme probes at ambient temperatures.RNA (New York, N.Y.) · 2020Article
- Toward a Rational Approach to Design Split G-Quadruplex Probes.ACS chemical biology · 2019Article
- Label-Free Pathogen Detection by a Deoxyribozyme Cascade with Visual Signal Readout.Sensors and actuators. B, Chemical · 2019Article
- FaptaSyme: A Strategy for Converting a Monomer/Oligomer-Nonselective Aptameric Sensor into an Oligomer-Selective One.Chembiochem : a European journal of chemical biology · 2018Article
- Nanoreactors based on DNAzyme-functionalized magnetic nanoparticles activated by magnetic field.Nanoscale · 2018Article
- A mutation-resistant deoxyribozyme OR gate for highly selective detection of viral nucleic acids.Chemical communications (Cambridge, England) · 2017Article
- Magnetic Field-Activated Sensing of mRNA in Living Cells.Journal of the American Chemical Society · 2017Article
- Multiplex detection of extensively drug resistant tuberculosis using binary deoxyribozyme sensors.Biosensors & bioelectronics · 2017Article
- Divide and Control: Comparison of Split and Switch Hybridization Sensors.ChemistrySelect · 2017Article
- DNA Computing Systems Activated by Electrochemically-triggered DNA Release from a Polymer-brush-modified Electrode Array.Electroanalysis · 2017Article
- DNA nanotechnology for nucleic acid analysis: multifunctional molecular DNA machine for RNA detection.Chemical communications (Cambridge, England) · 2016Article
- DNA Antenna Tile-Associated Deoxyribozyme Sensor with Improved Sensitivity.Chembiochem : a European journal of chemical biology · 2016Article
- Nonequilibrium Hybridization Enables Discrimination of a Point Mutation within 5-40 °C.Journal of the American Chemical Society · 2016Article
- Expedited quantification of mutant ribosomal RNA by binary deoxyribozyme (BiDz) sensors.RNA (New York, N.Y.) · 2015Article
- Bridging the Two Worlds: A Universal Interface between Enzymatic and DNA Computing Systems.Angewandte Chemie (International ed. in English) · 2015Article
- Divide and control: split design of multi-input DNA logic gates.Chemical communications (Cambridge, England) · 2015Article
- Deoxyribozyme cascade for visual detection of bacterial RNA.Chembiochem : a European journal of chemical biology · 2013Article
- Folding of 16S rRNA in a signal-producing structure for the detection of bacteria.Angewandte Chemie (International ed. in English) · 2013Article
- Mismatch Discrimination and Efficient Photomodulation with Split 10-23 DNAzymes.Inorganica chimica acta · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Along with biocompatibility, chemical stability, and simplicity of structural prediction and modification, deoxyribozyme-based molecular sensors have the potential of an improved detection limit due to their ability to catalytically amplify signal. This study contributes to the understanding of the factors responsible for the limit of detection (LOD) of RNA-cleaving deoxyribozyme sensors. A new sensor that detects specific DNA/RNA sequences was designed from deoxyribozyme OA-II [Chiuman, W.; Li, Y. (2006) J. Mol. Biol. 357, 748-754]. The sensor architecture allows for a unique combination of high selectivity, low LOD and the convenience of fluorescent signal monitoring in homogeneous solution. The LOD of the sensor was found to be approximately 1.6 x 10(-10) M after 3 h of incubation. An equation that allows estimation of the lowest theoretical LOD using characteristics of parent deoxyribozymes and their fluorogenic substrates was derived and experimentally verified. According to the equation, "catalytically perfect" enzymes can serve as scaffolds for the design of sensors with the LOD not lower than approximately 2 x 10(-15) M after 3 h of incubation. A new value termed the detection efficiency (DE) is suggested as a time-independent characteristic of a sensor's sensitivity. The expressions for the theoretical LOD and DE can be used to evaluate nucleic acid and protein enzymes for their application as biosensing platforms.
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Registered trials
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