ArticleInternational journal of legal medicine2010
Validation of adequate endogenous reference genes for the normalisation of qPCR gene expression data in human post mortem tissue.
Article in International journal of legal medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 78 citations in OpenAlex.
- Forensic Impact of the Omics Science Involved in the Wound: A Systematic Review.Frontiers in medicine · 2021Pooled it
- Temporal RT-qPCR-Based Porcine Cardiac Molecular Profiling for Post-Mortem Interval Estimation: Predictive Modeling.International journal of molecular sciences · 2026Article
- Molecular forensics: RNA degradation as a marker for postmortem interval determination.International journal of legal medicine · 2026Article
- Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR.Scientific reports · 2024Article
- A Strategy for the Selection of RT-qPCR Reference Genes Based on Publicly Available Transcriptomic Datasets.Biomedicines · 2023Article
- iNOS expressing macrophages co-localize with nitrotyrosine staining after myocardial infarction in humans.Frontiers in cardiovascular medicine · 2023Article
- Normalization strategy for selection of reference genes for RT-qPCR analysis in left ventricles of failing human hearts.BMC cardiovascular disorders · 2022Article
- Postmortem expression of apoptosis-related genes in the liver of mice and their use for estimation of the time of death.International journal of legal medicine · 2021Article
- MicroRNAs as Useful Tools to Estimate Time Since Death. A Systematic Review of Current Literature.Diagnostics (Basel, Switzerland) · 2021Review
- Changes in gene expression patterns in postmortem human myocardial infarction.International journal of legal medicine · 2020Article
- Quantitative analysis of noncoding RNA from paired fresh and formalin-fixed paraffin-embedded brain tissues.International journal of legal medicine · 2020Article
- Analysis of RNA in the estimation of post-mortem interval: a review of current evidence.International journal of legal medicine · 2019Review
- Neuromuscular Junction Changes in a Mouse Model of Charcot-Marie-Tooth Disease Type 4C.International journal of molecular sciences · 2018Article
- Evaluating the potential of housekeeping genes, rRNAs, snRNAs, microRNAs and circRNAs as reference genes for the estimation of PMI.Forensic science, medicine, and pathology · 2018Article
- Cell Death-Associated Ribosomal RNA Cleavage in Postmortem Tissues and Its Forensic Applications.Molecules and cells · 2017Article
- Increased cerebral expressions of MMPs, CLDN5, OCLN, ZO1 and AQPs are associated with brain edema following fatal heat stroke.Scientific reports · 2017Article
- Estimation of the human postmortem interval using an established rat mathematical model and multi-RNA markers.Forensic science, medicine, and pathology · 2017Article
- Article
- DNA and RNA profiling of excavated human remains with varying postmortem intervals.International journal of legal medicine · 2016Article
- Decreased mRNA levels of cardiac Cx43 and ZO1 in sudden cardiac death related to coronary atherosclerosis: a pilot study.International journal of legal medicine · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene expression analyses based on messenger RNA (mRNA) profiling require accurate data normalisation. When using endogenous reference genes, these have to be validated carefully. Therefore, we examined the transcript stability of 10 potential reference genes using quantitative real-time polymerase chain reaction: beta actin, 18S rRNA, glyceraldehyde-3-phosphate dehydrogenase, TATA box-binding protein, hypoxanthine phosphoribosyl-transferase I, beta-2-microglobulin, hydroxymethylbilane synthase, succinate dehydrogenase complex, subunit A, cyclophilin A and ubiquitin C. The aim of the current study was to assess which reference genes show stable mRNA levels in human post mortem cardiac muscle, skeletal muscle and brain tissue. Considering cardiac muscle tissue, CYCA and TBP were identified as the most stable while in skeletal muscle tissue, SDHA and TBP, and in brain tissue, SDHA and HMBS turned out to be the most stable. Furthermore, we recommend a minimum of four carefully validated endogenous control genes for reliable data normalisation in human post mortem tissue. Parameters influencing the stability of transcript amounts were found to be mainly the post mortem interval in cardiac muscle and skeletal muscle tissue and the donor's cause of death in skeletal muscle and brain samples. Further parameters like gender, age at death and body mass index were found to influence mRNA quantities in skeletal muscle only. The set of stable control genes identified in this study may be used in further studies if the composition of the samples is similar to the one used here.
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