ArticlePLoS genetics2010
HP1 recruitment in the absence of argonaute proteins in Drosophila.
Article in PLoS genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
55 citing papers in PubMed, 71 citations in OpenAlex.
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- NURF301 contributes to gypsy chromatin insulator-mediated nuclear organization.Nucleic acids research · 2022Article
- Transposable Element Expression and Regulation Profile in Gonads of Interspecific Hybrids ofCells · 2021Article
- Genetics and molecular biology of male infertility among Iranian population: an update.American journal of translational research · 2021Review
- The zinc-finger protein CLAMP promotesJournal of cell science · 2019Article
- PIWI-interacting RNAs: small RNAs with big functions.Nature reviews. Genetics · 2019Review
- Maelstrom Represses Canonical Polymerase II Transcription within Bi-directional piRNA Clusters in Drosophila melanogaster.Molecular cell · 2019Article
- Investigation of piwi-interacting RNA pathway genes role in idiopathic non-obstructive azoospermia.Scientific reports · 2018Article
- Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells.Nucleic acids research · 2017Article
- Silencing of Transposable Elements by piRNAs in Drosophila: An Evolutionary Perspective.Genomics, proteomics & bioinformatics · 2017Review
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- Production of Small Noncoding RNAs from the flamenco Locus Is Regulated by the gypsy Retrotransposon of Drosophila melanogaster.Genetics · 2016Article
- Reexamining the P-Element Invasion of Drosophila melanogaster Through the Lens of piRNA Silencing.Genetics · 2016Review
- Maintenance of a Drosophila melanogaster Population Cage.Journal of visualized experiments : JoVE · 2016Article
- Targeting of P-Element Reporters to Heterochromatic Domains by Transposable Element 1360 in Drosophila melanogaster.Genetics · 2016Article
- Increased production of piRNAs from euchromatic clusters and genes in Anopheles gambiae compared with Drosophila melanogaster.Epigenetics & chromatin · 2015Article
- Regulation of histone methylation by noncoding RNAs.Biochimica et biophysica acta · 2014Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Highly repetitive and transposable element rich regions of the genome must be stabilized by the presence of heterochromatin. A direct role for RNA interference in the establishment of heterochromatin has been demonstrated in fission yeast. In metazoans, which possess multiple RNA-silencing pathways that are both functionally distinct and spatially restricted, whether RNA silencing contributes directly to heterochromatin formation is not clear. Previous studies in Drosophila melanogaster have suggested the involvement of both the AGO2-dependent endogenous small interfering RNA (endo-siRNA) as well as Piwi-interacting RNA (piRNA) silencing pathways. In order to determine if these Argonaute genes are required for heterochromatin formation, we utilized transcriptional reporters and chromatin immunoprecipitation of the critical factor Heterochromatin Protein 1 (HP1) to monitor the heterochromatic state of piRNA clusters, which generate both endo-siRNAs and the bulk of piRNAs. Surprisingly, we find that mutation of AGO2 or piwi increases silencing at piRNA clusters corresponding to an increase of HP1 association. Furthermore, loss of piRNA production from a single piRNA cluster results in genome-wide redistribution of HP1 and reduction of silencing at a distant heterochromatic site, suggesting indirect effects on HP1 recruitment. Taken together, these results indicate that heterochromatin forms independently of endo-siRNA and piRNA pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.