ArticlePLoS genetics2010
Drosophila Xpd regulates Cdk7 localization, mitotic kinase activity, spindle dynamics, and chromosome segregation.
Article in PLoS genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 42 citations in OpenAlex.
- Inhibition of scheggia/SLC25A1 citrate transporter alleviates XPD deficits.Scientific reports · 2025Article
- Mms19 promotes spindle microtubule assembly in Drosophila neural stem cells.PLoS genetics · 2020Article
- Moonlighting in Mitosis: Analysis of the Mitotic Functions of Transcription and Splicing Factors.Cells · 2020Review
- Review
- Nucleotide excision repair genes shaping embryonic development.Open biology · 2019Review
- Gene modification by fast-track recombineering for cellular localization and isolation of components of plant protein complexes.The Plant journal : for cell and molecular biology · 2019Article
- Xeroderma Pigmentosum Group D (XPD) Inhibits the Proliferation Cycle of Vascular Smooth Muscle Cell (VSMC) by Activating Glycogen Synthase Kinase 3β (GSK3β).Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- Article
- A CK1 FRET biosensor reveals that DDX3X is an essential activator of CK1ε.Journal of cell science · 2018Article
- Iron Sulfur and Molybdenum Cofactor Enzymes Regulate theFrontiers in physiology · 2018Article
- The genetic basis for variation in resistance to infection in the Drosophila melanogaster genetic reference panel.PLoS pathogens · 2017Article
- Conservation and Divergence in Nucleotide Excision Repair Lesion Recognition.The Journal of biological chemistry · 2016Article
- TFIIH: New Discoveries Regarding its Mechanisms and Impact on Cancer Treatment.Journal of Cancer · 2016Review
- Article
- Fission yeast Cdk7 controls gene expression through both its CAK and C-terminal domain kinase activities.Molecular and cellular biology · 2015Article
- Unique subcellular distribution of RPB1 with a phosphorylated C-terminal domain (CTD) in mouse oocytes during meiotic division and its relationship with chromosome separation.The Journal of reproduction and development · 2015Article
- Crumbs and Xpd in mitosis.Oncoscience · 2015Article
- A Drosophila XPD model links cell cycle coordination with neuro-development and suggests links to cancer.Disease models & mechanisms · 2015Article
- Double-sieving-defective aminoacyl-tRNA synthetase causes protein mistranslation and affects cellular physiology and development.Nature communications · 2014Article
- Argonaute-1 functions as a mitotic regulator by controlling Cyclin B during Drosophila early embryogenesis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The trimeric CAK complex functions in cell cycle control by phosphorylating and activating Cdks while TFIIH-linked CAK functions in transcription. CAK also associates into a tetramer with Xpd, and our analysis of young Drosophila embryos that do not require transcription now suggests a cell cycle function for this interaction. xpd is essential for the coordination and rapid progression of the mitotic divisions during the late nuclear division cycles. Lack of Xpd also causes defects in the dynamics of the mitotic spindle and chromosomal instability as seen in the failure to segregate chromosomes properly during ana- and telophase. These defects appear to be also nucleotide excision repair (NER)-independent. In the absence of Xpd, misrouted spindle microtubules attach to chromosomes of neighboring mitotic figures, removing them from their normal location and causing multipolar spindles and aneuploidy. Lack of Xpd also causes changes in the dynamics of subcellular and temporal distribution of the CAK component Cdk7 and local mitotic kinase activity. xpd thus functions normally to re-localize Cdk7(CAK) to different subcellular compartments, apparently removing it from its cell cycle substrate, the mitotic Cdk. This work proves that the multitask protein Xpd also plays an essential role in cell cycle regulation that appears to be independent of transcription or NER. Xpd dynamically localizes Cdk7/CAK to and away from subcellular substrates, thereby controlling local mitotic kinase activity. Possibly through this activity, xpd controls spindle dynamics and chromosome segregation in our model system. This novel role of xpd should also lead to new insights into the understanding of the neurological and cancer aspects of the human XPD disease phenotypes.
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