ArticlePloS one2010
Levels of 17beta-estradiol receptors expressed in embryonic and adult zebrafish following in vivo treatment of natural or synthetic ligands.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 91 citations in OpenAlex.
- Dietary Glyphosate Exposure Disrupts Hepatic and Reproductive Function in Female Zebrafish at Regulatory Safe Levels.Toxics · 2026Article
- Suppression of brain aromatase decreases the expression of dopaminergic neuron in early development of zebrafish.Fish physiology and biochemistry · 2025Article
- Loss of adult visual responses by developmental BPA exposure is correlated with altered estrogenic signaling.Communications biology · 2025Article
- The use ofSystems biology in reproductive medicine · 2023Article
- The Zebrafish (International journal of molecular sciences · 2019Article
- Differential activity of BPA, BPAF and BPC on zebrafish estrogen receptors in vitro and in vivo.Toxicology and applied pharmacology · 2019Article
- Involvement of Estrogen and Its Receptors in Morphological Changes in the Eyes of the Japanese Eel,Cells · 2019Article
- Evaluation of the estrogen receptor alpha as a possible target of bifenthrin effects in the estrogenic and dopaminergic signaling pathways in zebrafish embryos.The Science of the total environment · 2019Article
- Brain aromatase modulates cardiac functions in embryonic zebrafish.International journal of veterinary science and medicine · 2019Article
- Immune-Specific Expression and Estrogenic Regulation of the Four Estrogen Receptor Isoforms in Female Rainbow Trout (Oncorhynchus mykiss).International journal of molecular sciences · 2018Article
- Early life exposure to ethinylestradiol enhances subsequent responses to environmental estrogens measured in a novel transgenic zebrafish.Scientific reports · 2018Article
- Estrogen receptor signaling during vertebrate development.Biochimica et biophysica acta · 2015Review
- Estrogen defines the dorsal-ventral limit of VEGF regulation to specify the location of the hemogenic endothelial niche.Developmental cell · 2014Article
- Morpholino-mediated knockdown of ERα, ERβa, and ERβb mRNAs in zebrafish (Danio rerio) embryos reveals differential regulation of estrogen-inducible genes.Endocrinology · 2013Article
- Hepatic and plasma sex differences in saturated and monounsaturated fatty acids are associated with differences in expression of elongase 6, but not stearoyl-CoA desaturase in Sprague-Dawley rats.Genes & nutrition · 2013Article
- Identification of estrogen target genes during zebrafish embryonic development through transcriptomic analysis.PloS one · 2013Article
- The zebrafish orthologue of the dyslexia candidate gene DYX1C1 is essential for cilia growth and function.PloS one · 2013Article
- Biosensor zebrafish provide new insights into potential health effects of environmental estrogens.Environmental health perspectives · 2012Article
- Regulation of thyroid hormone-, oestrogen- and androgen-related genes by triiodothyronine in the brain of Silurana tropicalis.Journal of neuroendocrinology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nuclear receptors encompass a group of regulatory proteins involved in a number of physiological processes. The estrogen receptors (ERs), of which one alpha and one beta form exist in mammals function as transcription factors in response to 17beta-estradiol (E2). In zebrafish there are three gene products of estrogen receptors and they are denoted esr1 (ERalpha), esr2a (ERbeta2) and esr2b (ERbeta1). Total RNA of zebrafish early life stages (<3, 6, 12, 24, 48, 72, 96 and 120 hours post fertilization) and of adult fish (liver, intestine, eye, heart, brain, ovary, testis, gill, swim bladder and kidney) were isolated following in vivo exposures. Using specific primers for each of the three zebrafish ERs the expression levels were quantified using real time PCR methodology. It was shown that in absence of exposure all three estrogen receptors were expressed in adult fish. The levels of expression of two of these three ER genes, the esr1 and esr2a were altered in organs such as liver, intestine, brain and testis in response to ligand (E2, diethylstilbestrol or 4-nonylphenol). During embryogenesis two of the three receptor genes, esr1 and esr2b were expressed, and in presence of ligand the mRNA levels of these two genes increased. The conclusions are i) estrogen receptor genes are expressed during early development ii) altered expression of esr genes in response to ligand is dependent on the cellular context; iii) the estrogenic ligand 4-nonylphenol, a manufactured compound commonly found in sewage of water treatment plants, acts as an agonist of the estrogen receptor during development and has both agonist and antagonist properties in tissues of adult fish. This knowledge of esr gene function in development and in adult life will help to understand mechanisms of interfering mimicking endocrine chemicals in vivo.
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