ArticleNucleic acids research2010
Engineering a family of synthetic splicing ribozymes.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- High-throughput engineering of ligand-activated splicing ribozyme through domain insertion.bioRxiv : the preprint server for biology · 2026Article
- Programming RNA Trans-Splicing for Versatile Gene Regulation and Complex Cellular Logic Computation.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Article
- Roles of long-range tertiary interactions in limiting dynamics of the Tetrahymena group I ribozyme.Journal of the American Chemical Society · 2014Article
- A role for a single-stranded junction in RNA binding and specificity by the Tetrahymena group I ribozyme.Journal of the American Chemical Society · 2012Article
- Computational design of synthetic regulatory networks from a genetic library to characterize the designability of dynamical behaviors.Nucleic acids research · 2011Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Controlling RNA splicing opens up possibilities for the synthetic biologist. The Tetrahymena ribozyme is a model group I self-splicing ribozyme that has been shown to be useful in synthetic circuits. To create additional splicing ribozymes that can function in synthetic circuits, we generated synthetic ribozyme variants by rationally mutating the Tetrahymena ribozyme. We present an alignment visualization for the ribozyme termed as structure information diagram that is similar to a sequence logo but with alignment data mapped on to secondary structure information. Using the alignment data and known biochemical information about the Tetrahymena ribozyme, we designed synthetic ribozymes with different primary sequences without altering the secondary structure. One synthetic ribozyme with 110 nt mutated retained 12% splicing efficiency in vivo. The results indicate that our biochemical understanding of the ribozyme is accurate enough to engineer a family of active splicing ribozymes with similar secondary structure but different primary sequences.
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