ArticleExperimental neurology2010
RNAi targeting micro-calpain increases neuron survival and preserves hippocampal function after global brain ischemia.
Article in Experimental neurology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- In Vivo Calpain Knockdown Using Delivery of siRNA.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Isoform-specific hyperactivation of calpain-2 occurs presymptomatically at the synapse in Alzheimer's disease mice and correlates with memory deficits in human subjects.Scientific reports · 2018Article
- Molecular chaperones and hypoxic-ischemic encephalopathy.Neural regeneration research · 2017Review
- Functional implications of axon initial segment cytoskeletal disruption in stroke.Acta pharmacologica Sinica · 2016Review
- Apurinic/apyrimidinic endonuclease 1 upregulation reduces oxidative DNA damage and protects hippocampal neurons from ischemic injury.Antioxidants & redox signaling · 2015Article
- Involvement of calpains in adult neurogenesis: implications for stroke.Frontiers in cellular neuroscience · 2015Article
- Role of calpains in the injury-induced dysfunction and degeneration of the mammalian axon.Neurobiology of disease · 2013Review
- Calpain 1 knockdown improves tissue sparing and functional outcomes after spinal cord injury in rats.Journal of neurotrauma · 2013Article
- A host GPCR signaling network required for the cytolysis of infected cells facilitates release of apicomplexan parasites.Cell host & microbe · 2013Article
- Gene delivery with viral vectors for cerebrovascular diseases.Frontiers in bioscience (Elite edition) · 2013Review
- IL-1RAcPb signaling regulates adaptive mechanisms in neurons that promote their long-term survival following excitotoxic insults.Frontiers in cellular neuroscience · 2013Article
- Temporal dependence of cysteine protease activation following excitotoxic hippocampal injury.Neuroscience · 2012Article
- Calpain 2 activated through N-methyl-D-aspartic acid receptor signaling cleaves CPEB3 and abrogates CPEB3-repressed translation in neurons.Molecular and cellular biology · 2012Article
- Calpastatin overexpression limits calpain-mediated proteolysis and behavioral deficits following traumatic brain injury.Experimental neurology · 2012Article
- RNA interference and ischemic injury.Molecular biology reports · 2012Review
- Ischemia-induced calpain activation causes eukaryotic (translation) initiation factor 4G1 (eIF4GI) degradation, protein synthesis inhibition, and neuronal death.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Calpains and delayed calcium deregulation in excitotoxicity.Neurochemical research · 2010Article
- Targeting individual calpain isoforms for neuroprotection.Experimental neurology · 2010Article
- Regulation of calpain-2 in neurons: implications for synaptic plasticity.Molecular neurobiology · 2010Review
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9 authors at 2 institutions in 1 country.
Funding
Abstract
The calpain family of cysteine proteases has a well-established causal role in neuronal cell death following acute brain injury. However, the relative contribution of calpain isoforms has not been determined in in vivo models. Identification of the calpain isoform responsible for neuronal injury is particularly important given the differential role of calpain isoforms in normal physiology. This study evaluates the role of m-calpain and micro-calpain in an in vivo model of global brain ischemia. Adeno-associated viral vectors expressing short hairpin RNAs targeting the catalytic subunits of micro- or m-calpain were used to knockdown expression of the targeted isoforms in adult rat hippocampal CA1 pyramidal neurons. Knockdown of micro-calpain, but not m-calpain, prevented calpain activity 72 h after 6-min transient forebrain ischemia, increased long-term survival and protected hippocampal electrophysiological function. These findings represent the first in vivo evidence that reducing expression of an individual calpain isoform can decrease post-ischemic neuronal death and preserve hippocampal function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.