ArticleThe Journal of cell biology1991
Endocytosis of junctional cadherins in bovine kidney epithelial (MDBK) cells cultured in low Ca2+ ion medium.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 174 citations in OpenAlex.
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- Late domain dependent E-cadherin recruitment into extracellular vesicles.Frontiers in cell and developmental biology · 2022Article
- CDHR3 extracellular domains EC1-3 mediate rhinovirus C interaction with cells and as recombinant derivatives, are inhibitory to virus infection.PLoS pathogens · 2018Article
- Homotypic endothelial nanotubes induced by wheat germ agglutinin and thrombin.Scientific reports · 2018Article
- Adherens Junctions on the Move-Membrane Trafficking of E-Cadherin.Cold Spring Harbor perspectives in biology · 2017Review
- E-cadherin interactome complexity and robustness resolved by quantitative proteomics.Science signaling · 2014Article
- PLEKHA7 modulates epithelial tight junction barrier function.Tissue barriers · 2014Article
- Characterization and Endocytic Internalization of Epith-2 Cell Surface Glycoprotein during the Epithelial-to-Mesenchymal Transition in Sea Urchin Embryos.Frontiers in endocrinology · 2013Article
- E-cadherin is critical for collective sheet migration and is regulated by the chemokine CXCL12 protein during restitution.The Journal of biological chemistry · 2012Article
- Adherens junction turnover: regulating adhesion through cadherin endocytosis, degradation, and recycling.Sub-cellular biochemistry · 2012Review
- The PIKfyve inhibitor YM201636 blocks the continuous recycling of the tight junction proteins claudin-1 and claudin-2 in MDCK cells.PloS one · 2012Article
- E-N-cadherin heterodimers define novel adherens junctions connecting endoderm-derived cells.The Journal of cell biology · 2011Article
- Connexin 43 is critical to maintain the homeostasis of the blood-testis barrier via its effects on tight junction reassembly.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Involvement of the interaction of afadin with ZO-1 in the formation of tight junctions in Madin-Darby canine kidney cells.The Journal of biological chemistry · 2010Article
- Article
- Adherens junction: molecular architecture and regulation.Cold Spring Harbor perspectives in biology · 2009Review
- Discovering the molecular components of intercellular junctions--a historical view.Cold Spring Harbor perspectives in biology · 2009Review
- Regulation of cadherin trafficking.Traffic (Copenhagen, Denmark) · 2009Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The release of intercellular contacts in MDBK cells, initiated by the depletion of Ca2+ ions from the culture medium, results in the endocytotic uptake of membrane vesicles containing specific membrane constituents of the zonula adhaerens (ZA). During this process the junction-derived, endocytosed vesicles remain associated with the ZA plaque components, while the plaque and its attached actin filaments retract as a whole in a ring-like fashion from the plasma membrane, often accumulating, usually in fragments, in the juxtanuclear cytoplasm. Double-label immunofluorescence microscopy with antiplakoglobin and antivinculin has indicated that both plaque proteins colocalize with the hallmark membrane glycoprotein of this junction type, E-cadherin (uvomorulin). When HRP used as a fluid phase marker is applied to the culture medium, simultaneously with the Ca2+ ion-chelator EGTA, numerous HRP-positive vesicles are found in close association with the dislocated plaque material, suggesting that the HRP is contained in the vesicles formed upon EGTA-induced junction splitting. Immunoelectron microscopy with various cadherin-specific antibodies revealed vesicle-associated labeling, confirming the derivation of these plaque-associated vesicles from the ZA. As the desmosome-specific cadherin, desmoglein, is recovered in another type of junction-derived vesicle, which is characterized by its association with a desmoplakin-plaque, we conclude that the membrane domains of both kinds of junction are endocytosed during Ca2+ depletion but stay in different vesicle populations, emphasizing the selective interaction of the specific cadherins with their respective plaque and filament partners.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.