ArticleThe EMBO journal1991
Multiple Ca2+/calmodulin-dependent protein kinase genes in a unicellular eukaryote.
Article in The EMBO journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 124 citations in OpenAlex.
- The calcineurin-responsive transcription factor Crz1 regulates the expression of CMK2 via a sole CDRE site in its promoter in budding yeast.Scientific reports · 2025Article
- Identification of the Beta Subunit Fas1p of Fatty Acid Synthetase as an Interacting Partner of Yeast Calcium/Calmodulin-Dependent Protein Kinase Cmk2p Through Mass Spectrometry Analysis.Applied biochemistry and biotechnology · 2024Article
- Characterization and Functional Analysis of a New Calcium/Calmodulin-Dependent Protein Kinase (CaMK1) in the Citrus Pathogenic FungusJournal of fungi (Basel, Switzerland) · 2022Article
- A kinase cascade on the yeast lysosomal vacuole regulates its membrane dynamics: conserved kinase Env7 is phosphorylated by casein kinase Yck3.The Journal of biological chemistry · 2020Article
- The protein kinase Cmk2 negatively regulates the calcium/calcineurin signalling pathway and expression of calcium pump genes PMR1 and PMC1 in budding yeast.Cell communication and signaling : CCS · 2019Article
- Functional wiring of the yeast kinome revealed by global analysis of genetic network motifs.Genome research · 2012Article
- Ste11p MEKK signals through HOG, mating, calcineurin and PKC pathways to regulate the FKS2 gene.BMC molecular biology · 2011Article
- Calcium/calmodulin kinase1 and its relation to thermotolerance and HSP90 in Sporothrix schenckii: an RNAi and yeast two-hybrid study.BMC microbiology · 2011Article
- Simultaneous genome-wide inference of physical, genetic, regulatory, and functional pathway components.PLoS computational biology · 2010Article
- Identification of a cell death pathway in Candida albicans during the response to pheromone.Eukaryotic cell · 2010Article
- Nonapoptotic death of Saccharomyces cerevisiae cells that is stimulated by Hsp90 and inhibited by calcineurin and Cmk2 in response to endoplasmic reticulum stresses.Eukaryotic cell · 2008Article
- Functional, genetic and bioinformatic characterization of a calcium/calmodulin kinase gene in Sporothrix schenckii.BMC microbiology · 2007Article
- Mechanisms regulating the protein kinases of Saccharomyces cerevisiae.Eukaryotic cell · 2007Review
- Dominant-negative mutant phenotypes and the regulation of translation elongation factor 2 levels in yeast.Nucleic acids research · 2005Article
- Binding of calmodulin to Nuf1p is required for karyogamy in Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2003Article
- Essential role of calcineurin in response to endoplasmic reticulum stress.The EMBO journal · 2002Article
- A homolog of voltage-gated Ca(2+) channels stimulated by depletion of secretory Ca(2+) in yeast.Molecular and cellular biology · 2000Article
- Induction of Ca2+-calmodulin signaling by hard-surface contact primes Colletotrichum gloeosporioides conidia to germinate and form appressoria.Journal of bacteriology · 1998Article
- Characterization of calcium/calmodulin-dependent protein kinase II activity in the nervous system of the lobster, Panulirus interruptus.Invertebrate neuroscience : IN · 1998Article
- Tcn1p/Crz1p, a calcineurin-dependent transcription factor that differentially regulates gene expression in Saccharomyces cerevisiae.Genes & development · 1997Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We purified a Ca2+/calmodulin (CaM)-dependent protein kinase (CaM kinase) from the yeast Saccharomyces cerevisiae with properties similar to mammalian type II CaM kinases. Degenerate oligonucleotides designed on the basis of the amino acid sequence of tryptic peptides from the 55 kd subunit of the yeast CaM kinase were used to isolate its gene from a set of lambda gt11-yeast genomic DNA phage clones initially selected by the ability to bind 125I-labelled yeast CaM. The cloned gene (CMK1) encodes an open reading frame that is homologous to the sequences of vertebrate type II CaM kinases. Several criteria demonstrated that the CMK1 gene product is the 55 kd polypeptide. Neither over-production (11-fold) nor complete elimination of the CMK1 gene product had any detectably deleterious effect on yeast cell growth. Extracts from cmk1 delta cells, which lacked detectable p55 using an antiserum raised against a Staphylococcus aureus protein A-CMK1 fusion protein, possessed significant residual Ca2+/CAM-dependent protein kinase activity. Using the CMK1 gene as a probe at low stringency, a second gene (CMK2) encoding another CaM-dependent protein kinase with striking sequence similarity to CMK1 was cloned. Deletion of CMK2, or both CMK1 and CMK2, was not lethal, although loss of CMK2 caused a slow rate of spore germination.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.