ArticleProceedings of the National Academy of Sciences of the United States of America1991
Expression of choline acetyltransferase mRNA in spermatogenic cells results in an accumulation of the enzyme in the postacrosomal region of mature spermatozoa.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 70 citations in OpenAlex.
- Molecular Diversity and Functional Implications of Mammalian Choline Acetyltransferases in Neuronal and Non-Neuronal Cells.International journal of molecular sciences · 2026Review
- The Smoky Impact of Nicotinic Acetylcholine Receptors on Testicular Function.Journal of clinical medicine · 2024Review
- Presence of key cholinergic enzymes in human spermatozoa and seminal fluid†.Biology of reproduction · 2024Article
- Elusive physiological role of prostatic acid phosphatase (PAP): generation of choline for sperm motility via auto-and paracrine cholinergic signaling.Frontiers in physiology · 2023Review
- Ldha-Dependent Metabolic Programs in Sertoli Cells Regulate Spermiogenesis in Mouse Testis.Biology · 2022Article
- Expression and Possible Role of Nicotinic Acetylcholine Receptor ε Subunit (AChRe) in Mouse Sperm.Biology · 2021Article
- Ameliorative effects ofVeterinary research forum : an international quarterly journal · 2017Article
- Ultrastructural localization and distribution of Nardilysin in mammalian male germ cells.Basic and clinical andrology · 2016Article
- Article
- Sperm epidermal growth factor receptor (EGFR) mediates α7 acetylcholine receptor (AChR) activation to promote fertilization.The Journal of biological chemistry · 2012Article
- Muscarinic acetylcholine receptor subtypes in the male reproductive tract: expression and function in rat efferent ductules and epididymis.Journal of molecular neuroscience : MN · 2010Review
- Immunohistochemical evidence suggests intrinsic regulatory activity of human eccrine sweat glands.Journal of anatomy · 1999Article
- Production and functional expression of an epitope-tagged human choline acetyltransferase.Journal of molecular neuroscience : MN · 1998Article
- Regulatory region in choline acetyltransferase gene directs developmental and tissue-specific expression in transgenic mice.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Human and monkey cholinergic neurons visualized in paraffin-embedded tissues by immunoreactivity for VAChT, the vesicular acetylcholine transporter.Journal of molecular neuroscience : MN · 1995Article
- Distribution of the vesicular acetylcholine transporter (VAChT) in the central and peripheral nervous systems of the rat.Journal of molecular neuroscience : MN · 1994Article
- Cloning of the rat gene encoding choline acetyltransferase, a cholinergic neuron-specific marker.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gene encoding choline acetyltransferase (ChAT; EC 2.3.1.6), the key enzyme in the synthesis of the neurotransmitter acetylcholine (ACh), is shown to be expressed in rat and human testes. High levels of two ChAT transcripts of 3.5 and 1.3 kilobases were detected by Northern blot analysis of adult rat testis RNA. A single ChAT mRNA species of 3.2 kilobases was detected in human testis. Cells responsible for the synthesis of ChAT mRNA in rat testis were localized by in situ hybridization in the middle part of the seminiferous epithelium, where the labeling was mostly found over spermatocytes and spermatids. Studies on the ontogeny of ChAT mRNA expression showed low levels in prepubertal rats with increasing levels as sexual maturation is reached. A peak of expression was seen at postnatal day 32, correlating with the onset of postmeiotic spermatogenesis. Results from surgical and pharmacological treatments suggest that androgens, as well as pituitary factors, could influence the relative levels of the two ChAT mRNAs detected in rat testis. Evidence for translation of the mRNA detected in the testis was obtained from the demonstration of ChAT-like immunoreactivity in ejaculated human spermatozoa. The staining was restricted to the postacrosomal region of the head, where the membrane of the sperm first fuses with that of the egg during fertilization, and to the annulus, a ring of dense material in the caudal end of the midpiece. Combined, these findings support the hypothesis that the neurotransmitter ACh is involved in reproductive function.
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