ArticleGene therapy2010
Indirect imaging of cardiac-specific transgene expression using a bidirectional two-step transcriptional amplification strategy.
Article in Gene therapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
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- In vivo bioimaging with tissue-specific transcription factor activated luciferase reporters.Scientific reports · 2015Article
- Susceptibility of murine induced pluripotent stem cell-derived cardiomyocytes to hypoxia and nutrient deprivation.Stem cell research & therapy · 2015Article
- A titratable two-step transcriptional amplification strategy for targeted gene therapy based on ligand-induced intramolecular folding of a mutant human estrogen receptor.Molecular imaging and biology · 2014Article
- Noninvasive imaging of hypoxia-inducible factor-1α gene therapy for myocardial ischemia.Human gene therapy methods · 2013Article
- Noninvasive monitoring of oxidative stress in transplanted mesenchymal stromal cells.JACC. Cardiovascular imaging · 2013Article
- In vivo bioluminescence imaging of cell differentiation in biomaterials: a platform for scaffold development.Tissue engineering. Part A · 2013Article
- A molecular imaging system based on both transcriptional and genomic amplification to detect prostate cancer cells in vivo.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Cell tracking and the development of cell-based therapies: a view from the Cardiovascular Cell Therapy Research Network.JACC. Cardiovascular imaging · 2012Review
- Optical imaging for stem cell differentiation to neuronal lineage.Nuclear medicine and molecular imaging · 2012Review
- Positron emission tomography reporter genes and reporter probes: gene and cell therapy applications.Theranostics · 2012Article
- Bioluminescence imaging: progress and applications.Trends in biotechnology · 2011Review
- Non-invasive bioluminescence imaging of myoblast-mediated hypoxia-inducible factor-1 alpha gene transfer.Molecular imaging and biology · 2011Article
- Experimental models to study lymphatic and blood vascular metastasis.Journal of surgical oncology · 2011Review
- Engineering and visualization of bacteria for targeting infarcted myocardium.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Article
- In vivo imaging of intraprostatic-specific gene transcription by PET.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2011Article
- Cardiovascular molecular imaging: focus on clinical translation.Circulation · 2011Review
- Longitudinal, noninvasive imaging of T-cell effector function and proliferation in living subjects.Cancer research · 2010Article
Corrections and comments
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Authors and funding
17 authors at 3 institutions in 1 country.
Funding
Abstract
Transcriptional targeting for cardiac gene therapy is limited by the relatively weak activity of most cardiac-specific promoters. We have developed a bidirectional plasmid vector, which uses a two-step transcriptional amplification (TSTA) strategy to enhance the expression of two optical reporter genes, firefly luciferase (fluc) and Renilla luciferase (hrluc), driven by the cardiac troponin T (cTnT) promoter. The vector was characterized in vitro and in living mice using luminometry and bioluminescence imaging to assess its ability to mediate strong, correlated reporter gene expression in a cardiac cell line and the myocardium, while minimizing expression in non-cardiac cell lines and the liver. In vitro, the TSTA system significantly enhanced cTnT-mediated reporter gene expression with moderate preservation of cardiac specificity. After intramyocardial and hydrodynamic tail vein delivery of an hrluc-enhanced variant of the vector, long-term fluc expression was observed in the heart, but not in the liver. In both the cardiac cell line and the myocardium, fluc expression correlated well with hrluc expression. These results show the vector's ability to effectively amplify and couple transgene expression in a cardiac-specific manner. Further replacement of either reporter gene with a therapeutic gene should allow non-invasive imaging of targeted gene therapy in living subjects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.