ArticleClinical and vaccine immunology : CVI2010
Consequences of interference of milk with chemoattractants for enzyme-linked immunosorbent assay quantifications.
Article in Clinical and vaccine immunology : CVI, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 15 citations in OpenAlex.
- A Single-Enzyme Electrochemical Biosensor for Lactose Detection in Human Breastmilk and Dairy Products.ACS measurement science au · 2026Article
- When is the best time to test paratuberculosis positivity? Observations from a follow-up study in Hungarian dairy herds.Frontiers in veterinary science · 2025Article
- Assessment of pregnancy-associated glycoprotein profile in milk for early pregnancy diagnosis in goats.Animal bioscience · 2021Article
- Article
- Antigen-Specific Mammary Inflammation Depends on the Production of IL-17A and IFN-γ by Bovine CD4+ T Lymphocytes.PloS one · 2015Article
- Impact of intramammary treatment on gene expression profiles in bovine Escherichia coli mastitis.PloS one · 2014Article
- T helper 17-associated cytokines are produced during antigen-specific inflammation in the mammary gland.PloS one · 2013Article
- Repertoire of Escherichia coli agonists sensed by innate immunity receptors of the bovine udder and mammary epithelial cells.Veterinary research · 2012Article
- Muramyl dipeptide synergizes with Staphylococcus aureus lipoteichoic acid to recruit neutrophils in the mammary gland and to stimulate mammary epithelial cells.Clinical and vaccine immunology : CVI · 2010Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Concentrations of the chemoattractants CXCL1, CXCL2, CXCL3, CXCL8, and C5a in milk were reduced by the preparation of milk whey by high-speed centrifugation or with rennet. About half of the chemoattractants (35 to 65%) were associated with the casein micelle sediment, except when whey was prepared by acidification. Consequently, quantification of chemoattractants should be carried out preferentially with skimmed milk samples or, whenever whey is needed, with acidic whey samples. The interference of milk or milk whey with the enzyme-linked immunosorbent assays (ELISAs) used to quantify the chemoattractants was moderate, as long as tetramethylbenzidine (TMB), not ABTS [2,2'-azino-bis-(3-ethylbenzthiazoline-sulfonate)], was used as the substrate of peroxidase. These considerations will help to assess more precisely a component of the immune response of the mammary gland to infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.