ArticleMolecular biology of the cell2010
Phosphatidylinositol 4,5-bisphosphate directs spermatid cell polarity and exocyst localization in Drosophila.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 51 citations in OpenAlex.
- TPTE, a testis-specific PTEN family member, drives spermatogenesis via PI(4,5)PCell death & disease · 2026Article
- Lipids in Insect Reproduction: Where, How, and Why.Advances in experimental medicine and biology · 2026Review
- The NEMP family supports metazoan fertility and nuclear envelope stiffness.Science advances · 2020Article
- Interplay between integrins and PI4P5K Sktl is crucial for cell polarization and reepithelialisation during Drosophila wound healing.Scientific reports · 2019Article
- Vangl2 regulates spermatid planar cell polarity through microtubule (MT)-based cytoskeleton in the rat testis.Cell death & disease · 2018Article
- Review
- The Role of Pnut and its Functional Domains inRussian journal of genetics. Applied research · 2017Article
- Phosphoinositide 5- and 3-phosphatase activities of a voltage-sensing phosphatase in living cells show identical voltage dependence.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Control of diverse subcellular processes by a single multi-functional lipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2].The Biochemical journal · 2016Review
- Establishing and maintaining cell polarity with mRNA localization in Drosophila.BioEssays : news and reviews in molecular, cellular and developmental biology · 2016Review
- Reduced expression of CDP-DAG synthase changes lipid composition and leads to male sterility in Drosophila.Open biology · 2016Article
- Exocyst-Dependent Membrane Addition Is Required for Anaphase Cell Elongation and Cytokinesis in Drosophila.PLoS genetics · 2015Article
- A dPIP5K dependent pool of phosphatidylinositol 4,5 bisphosphate (PIP2) is required for G-protein coupled signal transduction in Drosophila photoreceptors.PLoS genetics · 2015Article
- A migrating ciliary gate compartmentalizes the site of axoneme assembly in Drosophila spermatids.Current biology : CB · 2014Article
- Investigating spermatogenesis in Drosophila melanogaster.Methods (San Diego, Calif.) · 2014Review
- Spermatid cyst polarization in Drosophila depends upon apkc and the CPEB family translational regulator orb2.PLoS genetics · 2014Article
- Article
- Mutations in Cog7 affect Golgi structure, meiotic cytokinesis and sperm development during Drosophila spermatogenesis.Journal of cell science · 2012Article
- Eukaryotic initiation factor 4E-3 is essential for meiotic chromosome segregation, cytokinesis and male fertility in Drosophila.Development (Cambridge, England) · 2012Article
- A steep phosphoinositide bis-phosphate gradient forms during fungal filamentous growth.The Journal of cell biology · 2012Article
Corrections and comments
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Authors and funding
11 authors at 7 institutions in 4 countries.
Funding
Abstract
During spermiogenesis, Drosophila melanogaster spermatids coordinate their elongation in interconnected cysts that become highly polarized, with nuclei localizing to one end and sperm tail growth occurring at the other. Remarkably little is known about the signals that drive spermatid polarity and elongation. Here we identify phosphoinositides as critical regulators of these processes. Reduction of plasma membrane phosphatidylinositol 4,5-bisphosphate (PIP(2)) by low-level expression of the PIP(2) phosphatase SigD or mutation of the PIP(2) biosynthetic enzyme Skittles (Sktl) results in dramatic defects in spermatid cysts, which become bipolar and fail to fully elongate. Defects in polarity are evident from the earliest stages of elongation, indicating that phosphoinositides are required for establishment of polarity. Sktl and PIP(2) localize to the growing end of the cysts together with the exocyst complex. Strikingly, the exocyst becomes completely delocalized when PIP(2) levels are reduced, and overexpression of Sktl restores exocyst localization and spermatid cyst polarity. Moreover, the exocyst is required for polarity, as partial loss of function of the exocyst subunit Sec8 results in bipolar cysts. Our data are consistent with a mechanism in which localized synthesis of PIP(2) recruits the exocyst to promote targeted membrane delivery and polarization of the elongating cysts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.