ArticleJournal of clinical microbiology2010
Phages harboring specific peptides that recognize the N protein of the porcine reproductive and respiratory syndrome virus distinguish the virus from other viruses.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- Immune Protection Gap Between Porcine Reproductive and Respiratory Syndrome Subunit Vaccine (N Protein) and Live Vaccine.Vaccines · 2025Article
- Research progress on the N protein of porcine reproductive and respiratory syndrome virus.Frontiers in microbiology · 2024Review
- Establishment of a peptide-based enzyme-linked immunosorbent assay for detecting antibodies against PRRSV M protein.BMC veterinary research · 2021Article
- Damage to intestinal barrier integrity in piglets caused by porcine reproductive and respiratory syndrome virus infection.Veterinary research · 2021Article
- Article
- Putative phage-display epitopes of the porcine epidemic diarrhea virus S1 protein and their anti-viral activity.Virus genes · 2015Article
- Phage display for identifying peptides that bind the spike protein of transmissible gastroenteritis virus and possess diagnostic potential.Virus genes · 2015Article
- A Proline-Rich Domain in the Genotype 4 Hepatitis E Virus ORF3 C-Terminus Is Crucial for Downstream V105DLP108 Immunoactivity.PloS one · 2015Article
- A phage-displayed peptide recognizing porcine aminopeptidase N is a potent small molecule inhibitor of PEDV entry.Virology · 2014Article
- Transmissible gastroenteritis virus: identification of M protein-binding peptide ligands with antiviral and diagnostic potential.Antiviral research · 2013Article
- Phages bearing affinity peptides to severe acute respiratory syndromes-associated coronavirus differentiate this virus from other viruses.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2013Article
- Development of anti-infectives using phage display: biological agents against bacteria, viruses, and parasites.Antimicrobial agents and chemotherapy · 2012Review
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- Article
- Development of reverse transcription loop-mediated isothermal amplification for rapid detection of porcine epidemic diarrhea virus.Virus genes · 2011Article
- Reverse transcription loop-mediated isothermal amplification for rapid detection of transmissible gastroenteritis virus.Current microbiology · 2011Article
- Characterization and utility of monoclonal antibodies against spike protein of transmissible gastroenteritis virus.Letters in applied microbiology · 2011Article
- Phage displayed peptides recognizing porcine aminopeptidase N inhibit transmissible gastroenteritis coronavirus infection in vitro.Virology · 2011Article
- Development of a porcine epidemic diarrhea virus M protein-based ELISA for virus detection.Biotechnology letters · 2011Article
- Phage displayed peptides to avian H5N1 virus distinguished the virus from other viruses.PloS one · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of the current study was to develop a novel diagnostic test for detecting porcine reproductive and respiratory syndrome virus (PRRSV) using phage display technology. The N gene of PRRSV isolate HH08 was cloned following reverse transcription-PCR. Sequence comparison indicated that the N gene shared 96.4% homology to that of North American PRRSV (isolate VR2332) and 35.5% with that of European PRRSV (isolate LV), indicating that the PRRSV isolate was related to the North American PRRSV genotype. The bacterially expressed N protein was used as a target in a biopanning process using a phage display random peptide library. Seven phages expressing different peptides had a specific binding activity with the N protein. The putative binding motifs were identified by DNA sequencing. More importantly, the selected phages harboring specific peptides that recognize the N protein of PRRSV were able to efficiently distinguish PRRSV from other viruses in enzyme-linked immunosorbent assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.