ArticlePlant physiology2010
RNA interference-mediated change in protein body morphology and seed opacity through loss of different zein proteins.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 119 citations in OpenAlex.
- From development to yield: genetic and molecular regulation of agronomic traits in maize seeds.Frontiers in plant science · 2026Review
- Breeding for colored quality protein popcorn with improved amino acid composition.Frontiers in plant science · 2026Article
- Decades' progress and prospects on maize functional genomics and molecular breeding.Science China. Life sciences · 2025Review
- Dynamics of accumulation of lysine and tryptophan, and kernel modification in opaque2, opaque16, and double mutant (opaque2/opaque16) genotypes during kernel development in maize.Journal of applied genetics · 2025Article
- Duckweed: a starch-hyperaccumulating plant under cultivation with a combination of nutrient limitation and elevated COFrontiers in plant science · 2025Article
- Identification of QTLs associated withFrontiers in plant science · 2025Article
- Editing the 19 kDa alpha-zein gene family generates non-opaque2-based quality protein maize.Plant biotechnology journal · 2024Article
- An ARF gene mutation creates flint kernel architecture in dent maize.Nature communications · 2024Article
- Down-Regulation of Rice Glutelin byInternational journal of molecular sciences · 2023Article
- Genetic and molecular understanding for the development of methionine-rich maize: a holistic approach.Frontiers in plant science · 2023Review
- Generation of multi-layered protein bodies inFrontiers in plant science · 2023Article
- Unprocessed wheat γ-gliadin reduces gluten accumulation associated with the endoplasmic reticulum stress and elevated cell death.The New phytologist · 2022Article
- Co-overexpression ofFrontiers in plant science · 2022Article
- Cytosolic malate dehydrogenase 4 modulates cellular energetics and storage reserve accumulation in maize endosperm.Plant biotechnology journal · 2020Article
- Genetic enhancement of essential amino acids for nutritional enrichment of maize protein quality through marker assisted selection.Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Carotenoids modulate kernel texture in maize by influencing amyloplast envelope integrity.Nature communications · 2020Article
- Seed-produced anti-globulin VHH-Fc antibodies retrieve globulin precursors in the insoluble fraction and modulate the Arabidopsis thaliana seed subcellular morphology.Plant molecular biology · 2020Article
- The regulation of zein biosynthesis in maize endosperm.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- Towards coeliac-safe bread.Plant biotechnology journal · 2020Article
- Long-read sequencing reveals genomic structural variations that underlie creation of quality protein maize.Nature communications · 2020Article
5 more citing papers are in PubMed but not listed here.
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Opaque or nonvitreous phenotypes relate to the seed architecture of maize (Zea mays) and are linked to loci that control the accumulation and proper deposition of storage proteins, called zeins, into specialized organelles in the endosperm, called protein bodies. However, in the absence of null mutants of each type of zein (i.e. alpha, beta, gamma, and delta), the molecular contribution of these proteins to seed architecture remains unclear. Here, a double null mutant for the delta-zeins, the 22-kD alpha-zein, the beta-zein, and the gamma-zein RNA interference (RNAi; designated as z1CRNAi, betaRNAi, and gammaRNAi, respectively) and their combinations have been examined. While the delta-zein double null mutant had negligible effects on protein body formation, the betaRNAi and gammaRNAi alone only cause slight changes. Substantial loss of the 22-kD alpha-zeins by z1CRNAi resulted in protein body budding structures, indicating that a sufficient amount of the 22-kD zeins is necessary for maintenance of a normal protein body shape. Among different mutant combinations, only the combined betaRNAi and gammaRNAi resulted in drastic morphological changes, while other combinations did not. Overexpression of alpha-kafirins, the homologues of the maize 22-kD alpha-zeins in sorghum (Sorghum bicolor), in the beta/gammaRNAi mutant failed to offset the morphological alterations, indicating that beta- and gamma-zeins have redundant and unique functions in the stabilization of protein bodies. Indeed, opacity of the beta/gammaRNAi mutant was caused by incomplete embedding of the starch granules rather than by reducing the vitreous zone.
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