ArticleNucleic acids research2010
Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 145 papers.
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Who cites it
145 citing papers in PubMed.
- Genetic engineering of cyanophages for harmful cyanobacterial bloom treatment-A mini review.Synthetic and systems biotechnology · 2027Review
- Cyanobacterial conversion of COChemical science · 2026Review
- Synthetic consortia towards photosynthetically derived acetate for heterotrophic production of 1-butanol.Applied microbiology and biotechnology · 2026Article
- A set of constitutive promoters with graded strengths for gene expression in diverse cyanobacterial strains.Applied and environmental microbiology · 2026Article
- A set of constitutive promoters with graded strengths for gene expression in diverse cyanobacterial strains.bioRxiv : the preprint server for biology · 2026Article
- Harnessing engineered cyanobacteria for next-generation therapeutics.Archives of microbiology · 2026Review
- CyanoConstruct: simple platform for cyanobacterial expression construct assembly and translational tuning.BMC biotechnology · 2026Article
- A molecular toolbox to modulate gene expression and protein secretion in the bacterial predator Bdellovibrio bacteriovorus.PLoS genetics · 2025Article
- Multichassis Expression of Cyanobacterial and Other Bacterial Biosynthetic Gene Clusters.ACS synthetic biology · 2025Article
- Establishing a Malonyl-CoA Biosensor for the Two Model CyanobacteriaACS synthetic biology · 2025Article
- Probing substrate water access through the O1 channel of Photosystem II by single site mutations and membrane inlet mass spectrometry.Photosynthesis research · 2025Article
- Outlook on Synthetic Biology-Driven Hydrogen Production: Lessons from Algal Photosynthesis Applied to Cyanobacteria.Energy & fuels : an American Chemical Society journal · 2025Review
- Determinants ofGenes · 2024Article
- Metabolic engineering of Synechocystis sp. PCC 6803 for the improved production of phenylpropanoids.Microbial cell factories · 2024Article
- Heterologous Production in theAntioxidants (Basel, Switzerland) · 2023Article
- The organization of the phycobilisome-photosystem I supercomplex depends on the ratio between two different phycobilisome linker proteins.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2023Article
- The current situations and limitations of genetic engineering in cyanobacteria: a mini review.Molecular biology reports · 2023Review
- Light and carbon: Synthetic biology toward new cyanobacteria-based living biomaterials.Materials today. Bio · 2023Article
- Mapping competitive pathways to terpenoid biosynthesis in Synechocystis sp. PCC 6803 using an antisense RNA synthetic tool.Microbial cell factories · 2023Article
- Current Metabolic Engineering Strategies for Photosynthetic Bioproduction in Cyanobacteria.Microorganisms · 2023Review
85 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Cyanobacteria are suitable for sustainable, solar-powered biotechnological applications. Synthetic biology connects biology with computational design and an engineering perspective, but requires efficient tools and information about the function of biological parts and systems. To enable the development of cyanobacterial Synthetic Biology, several molecular tools were developed and characterized: (i) a broad-host-range BioBrick shuttle vector, pPMQAK1, was constructed and confirmed to replicate in Escherichia coli and three different cyanobacterial strains. (ii) The fluorescent proteins Cerulean, GFPmut3B and EYFP have been demonstrated to work as reporter proteins in cyanobacteria, in spite of the strong background of photosynthetic pigments. (iii) Several promoters, like P(rnpB) and variants of P(rbcL), and a version of the promoter P(trc) with two operators for enhanced repression, were developed and characterized in Synechocystis sp. strain PCC6803. (iv) It was shown that a system for targeted protein degradation, which is needed to enable dynamic expression studies, is working in Synechocystis sp. strain PCC6803. The pPMQAK1 shuttle vector allows the use of the growing numbers of BioBrick parts in many prokaryotes, and the other tools herein implemented facilitate the development of new parts and systems in cyanobacteria.
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