ArticleNucleic acids research2010
Novel intronic microRNA represses zebrafish myf5 promoter activity through silencing dickkopf-3 gene.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 26 citations in OpenAlex.
- Single-cell transcriptome reveals insights into the development and function of the zebrafish ovary.eLife · 2022Article
- Poly(U)-specific endoribonuclease ENDOU promotes translation of human CHOP mRNA by releasing uORF element-mediated inhibition.The EMBO journal · 2021Article
- Dkk3 dependent transcriptional regulation controls age related skeletal muscle atrophy.Nature communications · 2018Article
- RNA-Seq reveals MicroRNA expression signature and genetic polymorphism associated with growth and muscle quality traits in rainbow trout.Scientific reports · 2017Article
- An anterior signaling center patterns and sizes the anterior neuroectoderm of the sea urchin embryo.Development (Cambridge, England) · 2016Article
- Zebrafish and Medaka: new model organisms for modern biomedical research.Journal of biomedical science · 2016Review
- Retinoic acid-dependent regulation of miR-19 expression elicits vertebrate axis defects.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- MicroRNA-3906 regulates fast muscle differentiation through modulating the target gene homer-1b in zebrafish embryos.PloS one · 2013Article
- Integration of canonical and noncanonical Wnt signaling pathways patterns the neuroectoderm along the anterior-posterior axis of sea urchin embryos.PLoS biology · 2013Article
- Zebrafish Dkk3a protein regulates the activity of myf5 promoter through interaction with membrane receptor integrin α6b.The Journal of biological chemistry · 2012Article
- Identification and mechanism of regulation of the zebrafish dorsal determinant.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- dickkopf-3-related gene regulates the expression of zebrafish myf5 gene through phosphorylated p38a-dependent Smad4 activity.The Journal of biological chemistry · 2011Article
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Authors and funding
6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
A strong, negative cis-element located at the first intron +502/+835 (I300) of zebrafish myf5 has been reported. To elucidate the molecular mechanism underlying this repression network, we microinjected zebrafish single-cell embryos with I300 RNA, resulting in the dramatic reduction of luciferase activity driven by the myf5 promoter. Within this I300 segment, we identified an intronic microRNA (miR-In300) located at +609/+632 and found that it was more highly expressed in the older mature somites than those newly formed, which negatively correlated with the distribution of zebrafish myf5 transcripts. We proved that miR-In300 suppressed the transcription of myf5 through abolishing myf5 promoter activity, and we subsequently identified the long isoform of the Dickkopf-3 gene (dkk3) as the target gene of miR-In300. We further found that injection of the dkk3-morpholinos (MOs) resulted in downregulation of myf5 transcripts in somites, whereas co-injection of myf5 mRNA with dkk3-MO1 enabled rescue of the defects induced by dkk3-MO1 alone. Finally, injection of miR-In300-MO enhanced both myf5 transcripts in somites and the level of Dkk3 protein in zebrafish embryos. Based on these findings, we concluded that miR-In300 binds to its target gene dkk3, which inhibits the translation of dkk3 mRNA and, in turn, suppresses zebrafish myf5 promoter activity.
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