ArticleThe Journal of biological chemistry2010
Sphingosine 1-phosphate and sphingosine kinase are involved in a novel signaling pathway leading to acrosomal exocytosis.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- DDI-4 induces a temperature-sensitive exocytotic remodeling during C. elegans spermiogenesis via nsun-2-dependent sphingosine signaling.PLoS genetics · 2026Article
- Lipid Signature of Motile Human Sperm: Characterization of Sphingomyelin, Ceramide, and Phospholipids with a Focus on Very Long Chain Polyunsaturated Fatty Acids.International journal of molecular sciences · 2025Article
- Novel insights into the lipid signalling in human spermatozoa.Human reproduction (Oxford, England) · 2025Review
- Sphingolipids and Male Reproductive Health: A Narrative Review of Their Roles in Spermatogenesis, Fertility, and Dysfunction.Reproductive sciences (Thousand Oaks, Calif.) · 2025Review
- Sphingolipids modulate redox signalling during human sperm capacitation.Human reproduction (Oxford, England) · 2025Article
- Lipid remodeling in acrosome exocytosis: unraveling key players in the human sperm.Frontiers in cell and developmental biology · 2024Review
- The pair ceramide 1-phosphate/ceramide kinase regulates intracellular calcium and progesterone-induced human sperm acrosomal exocytosis.Frontiers in cell and developmental biology · 2023Article
- Sperm Lipid Markers of Male Fertility in Mammals.International journal of molecular sciences · 2021Review
- Application of High-Throughput Assays to Examine Phospho-Modulation of the Late Steps of Regulated Exocytosis.High-throughput · 2017Article
- Metabolites involved in cellular communication among human cumulus-oocyte-complex and sperm during in vitro fertilization.Reproductive biology and endocrinology : RB&E · 2015Article
- ADP ribosylation factor 6 (ARF6) promotes acrosomal exocytosis by modulating lipid turnover and Rab3A activation.The Journal of biological chemistry · 2015Article
- Metabolomics analysis of seminal plasma in infertile males with kidney-yang deficiency: a preliminary study.Evidence-based complementary and alternative medicine : eCAM · 2015Article
- Roles of sphingosine-1-phosphate in reproduction.Reproductive sciences (Thousand Oaks, Calif.) · 2014Review
- Investigations of motility and fertilization potential in thawed cryopreserved mouse sperm from cold-stored epididymides.Cryobiology · 2014Article
- MARCKS protein is phosphorylated and regulates calcium mobilization during human acrosomal exocytosis.PloS one · 2013Article
- Rab27 and Rab3 sequentially regulate human sperm dense-core granule exocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Sphingosine-1-phosphate rapidly increases cortisol biosynthesis and the expression of genes involved in cholesterol uptake and transport in H295R adrenocortical cells.Molecular and cellular endocrinology · 2012Article
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Regulated secretion is a central issue for the specific function of many cells; for instance, mammalian sperm acrosomal exocytosis is essential for egg fertilization. Sphingosine 1-phosphate is a bioactive sphingolipid that regulates crucial physiological processes. Here we report that this lipid triggers acrosomal exocytosis in human sperm by a mechanism involving a G(i)-coupled receptor. Real-time imaging showed a remarkable increase of cytosolic calcium upon activation with sphingosine 1-phosphate and pharmacological experiments indicate that the process requires extracellular calcium influx through voltage and store-operated calcium channels and efflux from intracellular stores through inositol 1,4,5-trisphosphate-sensitive calcium channels. Sphingosine 1-phosphate-induced exocytosis requires phospholipase C and protein kinase C activation. We investigated possible sources of the lipid. Western blot indicates that sphingosine kinase 1 is present in spermatozoa. Indirect immunofluorescence showed that phorbol ester, a potent protein kinase C activator that can also trigger acrosomal exocytosis, redistributes sphingosine kinase 1 to the acrosomal region. Functional assays showed that phorbol ester-induced exocytosis depends on the activation of sphingosine kinase 1. Furthermore, incorporation of (32)P to sphingosine demonstrates that cells treated with the phorbol ester increase their sphingosine kinase activity that yields sphingosine 1-phosphate. We present here the first evidence indicating that human spermatozoa produce sphingosine 1-phosphate when challenged with an exocytic stimulus. These observations point to a new role of sphingosine 1-phosphate in a signaling cascade that facilitates acrosome reaction providing some clues about novel lipid molecules involved in exocytosis.
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