ArticleBMC genomics2010
Complementary transcriptomic, lipidomic, and targeted functional genetic analyses in cultured Drosophila cells highlight the role of glycerophospholipid metabolism in Flock House virus RNA replication.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 50 citations in OpenAlex.
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- The Puzzling Conservation and Diversification of Lipid Droplets from Bacteria to Eukaryotes.Results and problems in cell differentiation · 2020Article
- Antimicrobial Peptides as Potential Antiviral Factors in Insect Antiviral Immune Response.Frontiers in immunology · 2020Review
- An engineered mutant of a host phospholipid synthesis gene inhibits viral replication without compromising host fitness.The Journal of biological chemistry · 2019Article
- Targeting host lipid flows: Exploring new antiviral and antibiotic strategies.Cellular microbiology · 2019Review
- A Transgenic Flock House Virus Replicon Reveals an RNAi Independent Antiviral Mechanism Acting inG3 (Bethesda, Md.) · 2019Article
- Host Lipids in Positive-Strand RNA Virus Genome Replication.Frontiers in microbiology · 2019Review
- Dicer-2 Regulates Resistance and Maintains Homeostasis against Zika Virus Infection inJournal of immunology (Baltimore, Md. : 1950) · 2018Article
- Defense Mechanisms against Viral Infection inViruses · 2018Review
- Viral rewiring of cellular lipid metabolism to create membranous replication compartments.Current opinion in cell biology · 2017Review
- Different Metabolic Pathways Are Involved in Response of Saccharomyces cerevisiae to L-A and M Viruses.Toxins · 2017Article
- Advances in Myeloid-Like Cell Origins and Functions in the Model Organism Drosophila melanogaster.Microbiology spectrum · 2017Review
- The interplay between intestinal bacteria and host metabolism in health and disease: lessons from Drosophila melanogaster.Disease models & mechanisms · 2016Review
- Positive-strand RNA viruses stimulate host phosphatidylcholine synthesis at viral replication sites.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Dynamic changes in host-virus interactions associated with colony founding and social environment in fire ant queens (Solenopsis invicta).Ecology and evolution · 2016Article
- Review
- RNA virus replication depends on enrichment of phosphatidylethanolamine at replication sites in subcellular membranes.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundCellular membranes are crucial host components utilized by positive-strand RNA viruses for replication of their genomes. Published studies have suggested that the synthesis and distribution of membrane lipids are particularly important for the assembly and function of positive-strand RNA virus replication complexes. However, the impact of specific lipid metabolism pathways in this process have not been well defined, nor have potential changes in lipid expression associated with positive-strand RNA virus replication been examined in detail.
resultsIn this study we used parallel and complementary global and targeted approaches to examine the impact of lipid metabolism on the replication of the well-studied model alphanodavirus Flock House virus (FHV). We found that FHV RNA replication in cultured Drosophila S2 cells stimulated the transcriptional upregulation of several lipid metabolism genes, and was also associated with increased phosphatidylcholine accumulation with preferential increases in lipid molecules with longer and unsaturated acyl chains. Furthermore, targeted RNA interference-mediated downregulation of candidate glycerophospholipid metabolism genes revealed a functional role of several genes in virus replication. In particular, we found that downregulation of Cct1 or Cct2, which encode essential enzymes for phosphatidylcholine biosynthesis, suppressed FHV RNA replication.
conclusionThese results indicate that glycerophospholipid metabolism, and in particular phosphatidylcholine biosynthesis, plays an important role in FHV RNA replication. Furthermore, they provide a framework in which to further explore the impact of specific steps in lipid metabolism on FHV replication, and potentially identify novel cellular targets for the development of drugs to inhibit positive-strand RNA viruses.
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Registered trials
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