ArticleJournal of proteome research2010
Identification of cell surface glycoprotein markers for glioblastoma-derived stem-like cells using a lectin microarray and LC-MS/MS approach.
Article in Journal of proteome research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 73 citations in OpenAlex.
- Keratan Sulfate Is a Multifunctional Brain Glycosaminoglycan With Instructive Capabilities.Journal of neurochemistry · 2025Review
- Glycosaminoglycans, Instructive Biomolecules That Regulate Cellular Activity and Synaptic Neuronal Control of Specific Tissue Functional Properties.International journal of molecular sciences · 2025Review
- David M. Lubman-The University of Michigan-A retrospective in research.Mass spectrometry reviews · 2023Review
- Integrated Transcriptome Profiling Identifies Prognostic Hub Genes as Therapeutic Targets of Glioblastoma: Evidenced by Bioinformatics Analysis.ACS omega · 2022Article
- Review
- Glycan characteristics of human heart constituent cells maintaining organ function: relatively stable glycan profiles in cellular senescence.Biogerontology · 2021Article
- A perspective on the radiopharmaceutical requirements for imaging and therapy of glioblastoma.Theranostics · 2021Review
- Immune Remodeling of the Extracellular Matrix Drives Loss of Cancer Stem Cells and Tumor Rejection.Cancer immunology research · 2020Article
- Down-regulation of tenascin-C inhibits breast cancer cells development by cell growth, migration, and adhesion impairment.PloS one · 2020Article
- A tension-mediated glycocalyx-integrin feedback loop promotes mesenchymal-like glioblastoma.Nature cell biology · 2018Article
- Metformin and temozolomide, a synergic option to overcome resistance in glioblastoma multiforme models.Oncotarget · 2017Article
- Cancer stem cell marker glycosylation: Nature, function and significance.Glycoconjugate journal · 2017Review
- Plant Lectins Targeting O-Glycans at the Cell Surface as Tools for Cancer Diagnosis, Prognosis and Therapy.International journal of molecular sciences · 2017Review
- Whether CD44 is an applicable marker for glioma stem cells.American journal of translational research · 2017Article
- A lectin-based cell microarray approach to analyze the mammalian granulosa cell surface glycosylation profile.Glycoconjugate journal · 2016Article
- Living Cell Microarrays: An Overview of Concepts.Microarrays (Basel, Switzerland) · 2016Review
- Tumor microenvironment tenascin-C promotes glioblastoma invasion and negatively regulates tumor proliferation.Neuro-oncology · 2016Article
- Dedifferentiation of patient-derived glioblastoma multiforme cell lines results in a cancer stem cell-like state with mitogen-independent growth.Journal of cellular and molecular medicine · 2015Article
- Lectin RCA-I specifically binds to metastasis-associated cell surface glycans in triple-negative breast cancer.Breast cancer research : BCR · 2015Article
- Tenascin-C: a novel candidate marker for cancer stem cells in glioblastoma identified by tissue microarrays.Journal of proteome research · 2015Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Despite progress in the treatment of glioblastoma, more than 95% of patients suffering from this disease still die within 2 years. Recent findings support the belief that cancer stem-like cells are responsible for tumor formation and ongoing growth. Here a method combining lectin microarray and LC-MS/MS was used to discover the cell surface glycoprotein markers of a glioblastoma-derived stem-like cell line. Lectin microarray analysis of cell surface glycans showed that two galactose-specific lectins Trichosanthes kirilowii agglutinin (TKA) and Peanut agglutinin (PNA) could distinguish the stem-like glioblastoma neurosphere culture from a traditional adherent glioblastoma cell line. Agarose-bound TKA and PNA were used to capture the glycoproteins from the two cell cultures, which were analyzed by LC-MS/MS. The glycoproteins were quantified by spectral counting, resulting in the identification of 12 and 11 potential glycoprotein markers from the TKA and PNA captured fractions respectively. Almost all of these proteins were membrane proteins. Differential expression was verified by Western blotting analysis of 6 interesting proteins, including the up-regulated Receptor-type tyrosine-protein phosphatase zeta, Tenascin-C, Chondroitin sulfate proteoglycan NG2, Podocalyxin-like protein 1 and CD90, and the down-regulated CD44. An improved understanding of these proteins may be important for earlier diagnosis and better therapeutic targeting of glioblastoma.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.