ArticlePediatric research2010
Macrophages are targeted by rotavirus in experimental biliary atresia and induce neutrophil chemotaxis by Mip2/Cxcl2.
Article in Pediatric research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 63 citations in OpenAlex.
- Gene expression signature for biliary atresia and a role for interleukin-8 in pathogenesis of experimental disease.Hepatology (Baltimore, Md.) · 2014Trial
- IL‑17AInternational journal of molecular medicine · 2026Article
- Dendritic cell expression of MyD88 is required for rotavirus-induced B cell activation.Journal of virology · 2025Article
- Transcriptional analysis of murine biliary atresia identifies macrophage heterogeneity and subset-specific macrophage functions.Frontiers in immunology · 2025Article
- Immunology of Biliary Atresia.Seminars in pediatric surgery · 2024Review
- Single-cell sequencing of a novel model of neonatal bile duct ligation in mice identifies macrophage heterogeneity in obstructive cholestasis.Scientific reports · 2023Article
- Necroptosis of macrophage is a key pathological feature in biliary atresia via GDCA/S1PR2/ZBP1/p-MLKL axis.Cell death & disease · 2023Article
- Current Understanding in the Clinical Characteristics and Molecular Mechanisms in Different Subtypes of Biliary Atresia.International journal of molecular sciences · 2022Review
- Article
- Identification and functional analysis of novel oncogene DDX60L in pancreatic ductal adenocarcinoma.BMC genomics · 2021Article
- RRV-induced biliary atresia in neonatal mice involves CD8 + T lymphocyte killer cells and the Notch signaling pathway.Genes & genomics · 2021Article
- Enteric viruses evoke broad host immune responses resembling those elicited by the bacterial microbiome.Cell host & microbe · 2021Article
- Rodent models of cholestatic liver disease: A practical guide for translational research.Liver international : official journal of the International Association for the Study of the Liver · 2021Review
- Characteristics ofThe Journal of international medical research · 2020Article
- The relative abundance of monocyte subsets determines susceptibility to perinatal hepatic inflammation.Journal of clinical & cellular immunology · 2020Article
- Innate Immunity and Pathogenesis of Biliary Atresia.Frontiers in immunology · 2020Review
- Tumour Necrosis Factor Alpha in Intestinal Homeostasis and Gut Related Diseases.International journal of molecular sciences · 2019Review
- Myeloid Cells during Viral Infections and Inflammation.Viruses · 2019Review
- Role of viruses in biliary atresia: news from mice and men.Innovative surgical sciences · 2018Review
- Review
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Biliary atresia is an obstructive cholangiopathy of unknown etiology. Although the adaptive immune system has been shown to regulate the obstruction of bile ducts in a rotavirus-induced mouse model, little is known about the virus-induced inflammatory response. Here, we hypothesized that cholangiocytes secrete chemoattractants in response to rotavirus. To test this hypothesis, we infected cholangiocyte and macrophage cell lines with rhesus rotavirus type A (RRV), quantified cytokines and chemokines and measured the migration of splenocytes. We also used PCR and immunostaining to search for new cellular targets of RRV in the liver. We found that RRV-infected cholangiocytes induced the mRNA expression for chemokines, but conditioned media failed to promote chemotaxis of splenocytes. Analyzing livers after viral challenge, we detected RRV in hepatic macrophages and demonstrated that media from RRV-infected macrophages have high concentrations of cytokines and chemokines and induced chemotaxis of neutrophils. Most notably, addition of anti-Mip2/Cxcl2 antibodies depleted this chemokine in the conditioned media and completely prevented neutrophil chemotaxis. In conclusion, infected cholangiocytes did not promote chemotaxis of inflammatory cells. Investigating alternate cellular targets of RRV, we detected the virus in hepatic macrophages and found that infected macrophages promoted neutrophil chemotaxis by release of Mip2/Cxcl2 in response to RRV.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.