ArticleBMC microbiology2010
Interactions between endocarditis-derived Streptococcus gallolyticus subsp. gallolyticus isolates and human endothelial cells.
Article in BMC microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- The evolving story ofFrontiers in microbiology · 2026Review
- Systematic analysis of the type VII secretion system inFrontiers in microbiology · 2026Article
- Posterior reversible encephalopathy syndrome in adult acute post-streptococcal glomerulonephritis.The Journal of international medical research · 2025Article
- A virological view of tenascin-C in infection.American journal of physiology. Cell physiology · 2023Review
- Microbiome and colorectal carcinogenesis: Linked mechanisms and racial differences.World journal of gastrointestinal oncology · 2022Review
- Potential involvement of Streptococcus mutans possessing collagen binding protein Cnm in infective endocarditis.Scientific reports · 2020Article
- Is Echocardiography Mandatory for AllInfection and drug resistance · 2020Article
- Neonatal calf meningitis associated with Streptococcus gallolyticus subsp. gallolyticus.Folia microbiologica · 2019Article
- A complex interplay between the extracellular matrix and the innate immune response to microbial pathogens.Immunology · 2018Review
- The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection.Scientific reports · 2018Article
- The Road to Infection: Host-Microbe Interactions Defining the Pathogenicity ofFrontiers in microbiology · 2018Review
- Biofilm formation and transcriptome analysis of Streptococcus gallolyticus subsp. gallolyticus in response to lysozyme.PloS one · 2018Article
- Strain-dependent interactions of Streptococcus gallolyticus subsp. gallolyticus with human blood cells.BMC microbiology · 2017Article
- Complete Genome Sequence of theGenome announcements · 2017Article
- Getting to the heart of the matter: Role of Streptococcus mutans adhesin Cnm in systemic disease.Virulence · 2017Article
- Transcriptome analysis of Streptococcus gallolyticus subsp. gallolyticus in interaction with THP-1 macrophage-like cells.PloS one · 2017Article
- Article
- New Insights into the Enterococcus faecium and Streptococcus gallolyticus subsp. gallolyticus Host Interaction Mechanisms.PloS one · 2016Article
- Streptococcus gallolyticus subsp. gallolyticus from human and animal origins: genetic diversity, antimicrobial susceptibility, and characterization of a vancomycin-resistant calf isolate carrying a vanA-Tn1546-like element.Antimicrobial agents and chemotherapy · 2015Article
- Rare and persistent Rhodococcus equi infection in a diffuse large B cell lymphoma patient: case report and review of the literature.Journal of thoracic disease · 2014Article
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4 authors.
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Abstract
backgroundStreptococcus gallolyticus subsp. gallolyticus is an important causative agent of infective endocarditis (IE) but the knowledge on virulence factors is limited and the pathogenesis of the infection is poorly understood. In the present study, we established an experimental in vitro IE cell culture model using EA.hy926 and HUVEC cells to investigate the adhesion and invasion characteristics of 23 Streptococcus gallolyticus subsp. gallolyticus strains from different origins (human IE-derived isolates, other human clinical isolates, animal isolates). Adhesion to eight components of the extracellular matrix (ECM) and the ability to form biofilms in vitro was examined in order to reveal features of S. gallolyticus subsp. gallolyticus endothelial infection. In addition, the strains were analyzed for the presence of the three virulence factors gtf, pilB, and fimB by PCR.
resultsThe adherence to and invasion characteristics of the examined S. gallolyticus subsp. gallolyticus strains to the endothelial cell line EA.hy926 differ significantly among themselves. In contrast, the usage of three different in vitro models (EA.hy926 cells, primary endothelial cells (HUVECs), mechanical stretched cells) revealed no differences regarding the adherence to and invasion characteristics of different strains. Adherence to the ECM proteins collagen I, II and IV revealed the highest values, followed by fibrinogen, tenascin and laminin. Moreover, a strong correlation was observed in binding to these proteins by the analyzed strains. All strains show the capability to adhere to polystyrole surfaces and form biofilms. We further confirmed the presence of the genes of two known virulence factors (fimB: all strains, gtf: 19 of 23 strains) and demonstrated the presence of the gene of one new putative virulence factor (pilB: 9 of 23 strains) by PCR.
conclusionOur study provides the first description of S. gallolyticus subsp. gallolyticus adhesion and invasion of human endothelial cells, revealing important initial information of strain variability, behaviour and characteristics of this as yet barely analyzed pathogen.
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