ArticleMolecular microbiology2010
A localized multimeric anchor attaches the Caulobacter holdfast to the cell pole.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 68 citations in OpenAlex.
- Sticking to Green: Sustainable Solutions for Next-Generation Microelectronic Packaging with Plant-Derived and Bacterial Adhesives.ACS sustainable chemistry & engineering · 2025Review
- Phase separation modulates the assembly and dynamics of a polarity-related scaffold-signaling hub.Nature communications · 2022Article
- What Glues the Glue to the Cell Surface?Journal of bacteriology · 2022Article
- HfaE Is a Component of the Holdfast Anchor Complex That Tethers the Holdfast Adhesin to the Cell Envelope.Journal of bacteriology · 2022Article
- Dual adhesive unipolar polysaccharides synthesized by overlapping biosynthetic pathways in Agrobacterium tumefaciens.Molecular microbiology · 2022Article
- Plasmid-Encoded H-NS Controls Extracellular Matrix Composition in a ModernJournal of bacteriology · 2021Article
- A polysaccharide deacetylase enhances bacterial adhesion in high-ionic-strength environments.iScience · 2021Article
- Article
- Comparative Analysis of Ionic Strength Tolerance between Freshwater and MarineJournal of bacteriology · 2019Article
- A Multiprotein Complex Anchors Adhesive Holdfast at the Outer Membrane of Caulobacter crescentus.Journal of bacteriology · 2019Article
- Composition of the Holdfast Polysaccharide fromJournal of bacteriology · 2019Article
- Feedback regulation of Caulobacter crescentus holdfast synthesis by flagellum assembly via the holdfast inhibitor HfiA.Molecular microbiology · 2018Article
- Article
- Mutations in Sugar-Nucleotide Synthesis Genes Restore Holdfast Polysaccharide Anchoring to Caulobacter crescentus Holdfast Anchor Mutants.Journal of bacteriology · 2018Article
- Cohesive Properties of themBio · 2017Article
- TonB-Dependent Heme/Hemoglobin Utilization by Caulobacter crescentus HutA.Journal of bacteriology · 2017Article
- A Rhizobiales-Specific Unipolar Polysaccharide Adhesin Contributes to Rhodopseudomonas palustris Biofilm Formation across Diverse Photoheterotrophic Conditions.Applied and environmental microbiology · 2017Article
- Proper Control of Caulobacter crescentus Cell Surface Adhesion Requires the General Protein Chaperone DnaK.Journal of bacteriology · 2016Article
- Timescales and Frequencies of Reversible and Irreversible Adhesion Events of Single Bacterial Cells.Analytical chemistry · 2015Article
- Adhesins Involved in Attachment to Abiotic Surfaces by Gram-Negative Bacteria.Microbiology spectrum · 2015Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Caulobacter crescentus attachment is mediated by the holdfast, a complex of polysaccharide anchored to the cell by HfaA, HfaB and HfaD. We show that all three proteins are surface exposed outer membrane (OM) proteins. HfaA is similar to fimbrial proteins and assembles into a high molecular weight (HMW) form requiring HfaD, but not holdfast polysaccharide. The HfaD HMW form is dependent on HfaA but not on holdfast polysaccharide. We show that HfaA and HfaD form homomultimers and that they require HfaB for stability and OM translocation. All three proteins localize to the late pre-divisional flagellar pole, remain at this pole in swarmer cells, and localize at the stalk tip after the stalk is synthesized at the same pole. Hfa protein localization requires the holdfast polysaccharide secretion proteins and the polar localization factor PodJ. An hfaB mutant is much more severely deficient in adherence and holdfast attachment than hfaA and hfaD mutants. An hfaA, hfaD double mutant phenocopies either single mutant, suggesting that HfaB is involved in holdfast attachment beyond secretion of HfaA and HfaD. We hypothesize that HfaB secretes HfaA and HfaD across the outer membrane, and the three proteins form a complex anchoring the holdfast to the stalk.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.