ArticlePLoS neglected tropical diseases2010
Ambient stable quantitative PCR reagents for the detection of Yersinia pestis.
Article in PLoS neglected tropical diseases, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Highly Sensitive Field Detection Technology for Anthrax Based on the CRISPR/Cas13a System.Microbial biotechnology · 2025Article
- Vacuum Oven Drying: A Cost-Effective Way of Producing Field-Deployable Reagents for In-house Real-Time PCR Methods.Molecular biotechnology · 2025Article
- Development and evaluation of a multi-target droplet digital PCR assay for highly sensitive and specific detection of Yersinia pestis.PLoS neglected tropical diseases · 2024Article
- Development of a non-infectious control for viral hemorrhagic fever PCR assays.PLoS neglected tropical diseases · 2024Article
- The Natural and Clinical History of Plague: From the Ancient Pandemics to Modern Insights.Microorganisms · 2024Review
- Article
- Yersinia pestis antibiotic resistance: a systematic review.Osong public health and research perspectives · 2022Article
- Development and evaluation of a multiplex droplet digital polymerase chain reaction method for simultaneous detection of five biothreat pathogens.Frontiers in microbiology · 2022Article
- Transferable, easy-to-use and room-temperature-storable PCR mixes for microfluidic molecular diagnostics.Talanta · 2021Article
- Pulse-Controlled Amplification-A new powerful tool for on-site diagnostics under resource limited conditions.PLoS neglected tropical diseases · 2021Article
- Yersinia pestis: the Natural History of Plague.Clinical microbiology reviews · 2020Review
- Long-term dry storage of enzyme-based reagents for isothermal nucleic acid amplification in a porous matrix for use in point-of-care diagnostic devices.The Analyst · 2020Article
- Calibration of an Upconverting Phosphor-Based Quantitative Immunochromatographic Assay for DetectingFrontiers in cellular and infection microbiology · 2020Article
- A novel ready-to-use dry-reagent polymerase chain reaction for detection ofThe Indian journal of medical research · 2019Article
- Plague: Recognition, Treatment, and Prevention.Journal of clinical microbiology · 2018Review
- Whole-Genome Sequencing in Microbial Forensic Analysis of Gamma-Irradiated Microbial Materials.Applied and environmental microbiology · 2016Article
- Yersinia pestis detection by loop-mediated isothermal amplification combined with magnetic bead capture of DNA.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
- Development of a dry-reagent mix-based polymerase chain reaction as a novel tool for the identification ofJournal of laboratory physiciansArticle
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlthough assays for detecting Yersinia pestis using TaqMan probe-based real-time PCR have been developed for years, little is reported on room-temperature-stable PCR reagents, which will be invaluable for field epidemic surveillance, immediate response to public health emergencies, counter-bioterrorism investigation, etc. In this work, a set of real-time PCR reagents for rapid detection of Y. pestis was developed with extraordinary stability at 37 degrees C. METHODS/PRINCIPAL
findingsTaqMan-based real-time PCR assays were developed using the primers and probes targeting the 3a sequence in the chromosome and the F1 antigen gene caf1 in the plasmid pMT1of Y. pestis, respectively. Then, carbohydrate mixtures were added to the PCR reagents, which were later vacuum-dried for stability evaluation. The vacuum-dried reagents were stable at 37 degrees C for at least 49 days for a lower concentration of template DNA (10 copies/microl), and up to 79 days for higher concentrations (> or =10(2) copies/microl). The reagents were used subsequently to detect soil samples spiked with Y. pestis vaccine strain EV76, and 5x10(4) CFU per gram of soil could be detected by both 3a- and caf1-based PCR reagents. In addition, a simple and efficient method for soil sample processing is presented here. CONCLUSIONS/SIGNIFICANCE: The vacuum-dried reagents for real-time PCR maintain accuracy and reproducibility for at least 49 days at 37 degrees C, indicating that they can be easily transported at room temperature for field application if the machine for performing real-time PCR is available. This dry reagent is of great significance for routine plague surveillance.
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Registered trials
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