ArticleJournal of visualized experiments : JoVE2010
Preparation of developing and adult Drosophila brains and retinae for live imaging.
Article in Journal of visualized experiments : JoVE, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Light- and temperature-sensitive seizures are regulated by spatially distinct cortex glial populations in the central nervous system.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Brain energy metabolism as an underlying basis of slow and fast cognitive phenotypes in honeybees.The Journal of experimental biology · 2024Article
- Stable Expression of dmiR-283 in the Brain Promises Positive Effects in Endurance Exercise on Sleep-Wake Behavior in AgingInternational journal of molecular sciences · 2023Article
- The disease-associated proteins Drosophila Nab2 and Ataxin-2 interact with shared RNAs and coregulate neuronal morphology.Genetics · 2022Article
- Dissections of Larval, Pupal and Adult Butterfly Brains for Immunostaining and Molecular Analysis.Methods and protocols · 2021Article
- Engineering of a bona fide light-operated calcium channel.Nature communications · 2021Article
- An Immobilization Technique for Long-Term Time-Lapse Imaging of ExplantedFrontiers in cell and developmental biology · 2020Article
- Immunocytochemical Labeling of Rhabdomeric Proteins inThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2019Article
- Neuronal overexpression of Alzheimer's disease and Down's syndrome associated DYRK1A/minibrain gene alters motor decline, neurodegeneration and synaptic plasticity in Drosophila.Neurobiology of disease · 2019Article
- Defective cortex glia plasma membrane structure underlies light-induced epilepsy inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains.Journal of visualized experiments : JoVE · 2018Article
- Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ.Journal of visualized experiments : JoVE · 2018Article
- A novel ex vivo method for measuring whole brain metabolism in model systems.Journal of neuroscience methods · 2018Article
- Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture.Journal of visualized experiments : JoVE · 2018Article
- Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains.Journal of visualized experiments : JoVE · 2017Article
- Single molecule fluorescence in situ hybridisation for quantitating post-transcriptional regulation in Drosophila brains.Methods (San Diego, Calif.) · 2017Article
- Analysis of axonal trafficking via a novel live-imaging technique reveals distinct hedgehog transport kinetics.Biology open · 2017Article
- In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila.Journal of visualized experiments : JoVE · 2016Article
- Wallenda/DLK protein levels are temporally downregulated by Tramtrack69 to allow R7 growth cones to become stationary boutons.Development (Cambridge, England) · 2016Article
- Filopodial dynamics and growth cone stabilization in Drosophila visual circuit development.eLife · 2015Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The Drosophila brain and visual system are widely utilized model systems to study neuronal development, function and degeneration. Here we show three preparations of the brain and visual system that cover the range from the developing eye disc-brain complex in the developing pupae to individual eye and brain dissection from adult flies. All protocols are optimized for the live culture of the preparations. However, we also present the conditions for fixed tissue immunohistochemistry where applicable. Finally, we show live imaging conditions for these preparations using conventional and resonant 4D confocal live imaging in a perfusion chamber. Together, these protocols provide a basis for live imaging on different time scales ranging from functional intracellular assays on the scale of minutes to developmental or degenerative processes on the scale of many hours.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.