ArticleInfection and immunity2010
Anaerobic metabolism occurs in the substratum of gonococcal biofilms and may be sustained in part by nitric oxide.
Article in Infection and immunity, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 46 citations in OpenAlex.
- Gentamicin induction of the gonococcalmBio · 2025Article
- Transcriptional responses ofmBio · 2024Article
- Dinner date: Neisseria gonorrhoeae central carbon metabolism and pathogenesis.Emerging topics in life sciences · 2024Review
- Meningococcal Urethritis: Old and New.Journal of clinical microbiology · 2022Review
- Transcriptional regulation of a gonococcal gene encoding a virulence factor (L-lactate permease).PLoS pathogens · 2019Article
- TheFrontiers in immunology · 2019Article
- Reduce, Induce, Thrive: Bacterial Redox Sensing during Pathogenesis.Journal of bacteriology · 2018Review
- Genome-resolved metagenomics of a bioremediation system for degradation of thiocyanate in mine water containing suspended solid tailings.MicrobiologyOpen · 2017Article
- Nitrate, nitrite and nitric oxide reductases: from the last universal common ancestor to modern bacterial pathogens.Current opinion in microbiology · 2016Review
- Examination of the Staphylococcus aureus nitric oxide reductase (saNOR) reveals its contribution to modulating intracellular NO levels and cellular respiration.Molecular microbiology · 2015Article
- Nitric oxide treatment for the control of reverse osmosis membrane biofouling.Applied and environmental microbiology · 2015Article
- Article
- Role of biofilm in children with recurrent upper respiratory tract infections.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Review
- Early effects of Staphylococcus aureus biofilm secreted products on inflammatory responses of human epithelial keratinocytes.Journal of inflammation (London, England) · 2014Article
- Identification of genes involved in Neisseria meningitidis colonization.Infection and immunity · 2013Article
- Characterization of an ntrX mutant of Neisseria gonorrhoeae reveals a response regulator that controls expression of respiratory enzymes in oxidase-positive proteobacteria.Journal of bacteriology · 2013Article
- Recombinant truncated AniA of pathogenic Neisseria elicits a non-native immune response and functional blocking antibodies.Biochemical and biophysical research communications · 2013Article
- Article
- Phenotypic characterization of a copA mutant of Neisseria gonorrhoeae identifies a link between copper and nitrosative stress.Infection and immunity · 2012Article
- Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Neisseria gonorrhoeae is the etiologic agent of gonorrhea, which has been among the most frequently reported communicable diseases in the United States since 1960. Women frequently do not exhibit symptoms, which can lead to chronic infection. N. gonorrhoeae readily forms biofilms over abiotic surfaces, over primary and transformed cervical epithelial cells, and over cervical tissues in vivo. Biofilms are often associated with chronic infection, which suggests a link between biofilm formation and asymptomatic gonorrhea in women. Proteins involved in anaerobic metabolism and oxidative-stress tolerance are critical for normal biofilm formation of N. gonorrhoeae. Therefore, we examined the spatial profiles of anaerobic respiration in N. gonorrhoeae, using an aniA'-'gfp transcriptional fusion. Nitric oxide (NO) can elicit biofilm dispersal when present at sublethal concentrations in the surrounding medium. Some reports indicate that NO may also encourage biofilm formation at higher, potentially lethal concentrations. NO is produced by polymorphonuclear lymphocytes (PMNs) and cervical endothelial and epithelial cells. Thus, we also examined the effect of NO on N. gonorrhoeae biofilms. We found that anaerobic respiration occurs predominantly in the substratum of gonococcal biofilms and that expression of aniA is induced over time in biofilms. Treatment with high concentrations of a rapid-release NO donor prevents biofilm formation when supplied early in biofilm development but can also enhance biofilm formation once anaerobic respiration is initiated. NO treatment partially restores biofilm formation in an aniA::kan insertion mutant, which suggests that N. gonorrhoeae in biofilms may use NO as a substrate for anaerobic growth but prefer nitrite.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.