ArticleInfection and immunity2010
Use of the Cre-lox recombination system to investigate the lp54 gene requirement in the infectious cycle of Borrelia burgdorferi.
Article in Infection and immunity, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Complex exchanges among plasmids and clonal expansion of lineages shape the population structure and virulence ofbioRxiv : the preprint server for biology · 2025Article
- Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme research.Scientific data · 2024Article
- Strict Conservation yet Non-Essential Nature of Plasmid GeneMicrobiology spectrum · 2023Article
- Structural Analysis of the Outer Membrane Lipoprotein BBA14 (OrfD) and the Corresponding Paralogous Gene Family 143 (PFam143) fromPathogens (Basel, Switzerland) · 2022Article
- Genetic Manipulation ofCurrent issues in molecular biology · 2021Review
- Structural and Functional Analysis of BBA03,Pathogens (Basel, Switzerland) · 2020Article
- Global Tn-seq analysis of carbohydrate utilization and vertebrate infectivity of Borrelia burgdorferi.Molecular microbiology · 2016Article
- Regulatory protein BBD18 of the lyme disease spirochete: essential role during tick acquisition?mBio · 2014Article
- Borrelia burgdorferi bba66 gene inactivation results in attenuated mouse infection by tick transmission.Infection and immunity · 2013Article
- Enhanced detection of host response antibodies to Borrelia burgdorferi using immuno-PCR.Clinical and vaccine immunology : CVI · 2013Article
- In vivo expression technology identifies a novel virulence factor critical for Borrelia burgdorferi persistence in mice.PLoS pathogens · 2013Article
- Borrelia burgdorferi and tick proteins supporting pathogen persistence in the vector.Future microbiology · 2013Review
- Competitive advantage of Borrelia burgdorferi with outer surface protein BBA03 during tick-mediated infection of the mammalian host.Infection and immunity · 2012Article
- Two systems for targeted gene deletion in Coxiella burnetii.Applied and environmental microbiology · 2012Article
- Genome stability of Lyme disease spirochetes: comparative genomics of Borrelia burgdorferi plasmids.PloS one · 2012Article
- Regulation of the virulence determinant OspC by bbd18 on linear plasmid lp17 of Borrelia burgdorferi.Journal of bacteriology · 2011Article
- Molecular characterization of the Borrelia burgdorferi in vivo-essential protein PncA.Microbiology (Reading, England) · 2011Article
- Borrelia burgdorferi linear plasmid 38 is dispensable for completion of the mouse-tick infectious cycle.Infection and immunity · 2011Article
- High-throughput plasmid content analysis of Borrelia burgdorferi B31 by using Luminex multiplex technology.Applied and environmental microbiology · 2011Article
- Genetic Manipulation of Borrelia Spp.Current topics in microbiology and immunologyReview
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Borrelia burgdorferi, the causative agent of Lyme disease, has a complex genome consisting of a linear chromosome and up to 21 linear and circular plasmids. These plasmids encode numerous proteins critical to the spirochete's infectious cycle and many hypothetical proteins whose functions and requirements are unknown. The conserved linear plasmid lp54 encodes several proteins important for survival in the mouse-tick infectious cycle, but the majority of the proteins are of unknown function and lack homologs outside the borreliae. In this study we adapted the Cre-lox recombination system to create large deletions in the B. burgdorferi genome. Using Cre-lox, we systematically investigated the contribution of 14 adjacent genes on the left arm of lp54 to the overall infectivity of B. burgdorferi. The deletion of the region of lp54 encompassing bba07 to bba14 had no significant effect on the infectious cycle of B. burgdorferi. The deletion of bba01 to bba07 resulted in a slight growth defect but did not significantly affect the ability of B. burgdorferi to complete the infectious cycle. This study demonstrated the utility of the Cre-lox system to efficiently explore gene requirements in B. burgdorferi and surprisingly revealed that a large number of the highly conserved proteins encoded on lp54 are not required to complete the infectious cycle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.