ArticleJournal of proteomics2010
A comparison of the accuracy of iTRAQ quantification by nLC-ESI MSMS and nLC-MALDI MSMS methods.
Article in Journal of proteomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- Review
- Label-free single cell proteomics utilizing ultrafast LC and MS instrumentation: A valuable complementary technique to multiplexing.Proteomics · 2023Review
- Transcriptome and iTRAQ-Based Proteome Reveal the Molecular Mechanism of Intestinal Injury Induced by Weaning Ewe's Milk in Lambs.Frontiers in veterinary science · 2022Article
- Differentiating Two Closely RelatedToxins · 2020Article
- Adverse effects of Δ9-tetrahydrocannabinol on neuronal bioenergetics during postnatal development.JCI insight · 2020Article
- Use of mTRAQ derivatization reagents on tissues for imaging neurotransmitters by MALDI imaging mass spectrometry: the triple spray method.Analytical and bioanalytical chemistry · 2019Article
- iTRAQ based proteomic analysis of PMRSC advances · 2019Article
- gpGrouper: A Peptide Grouping Algorithm for Gene-Centric Inference and Quantitation of Bottom-Up Proteomics Data.Molecular & cellular proteomics : MCP · 2018Article
- Real-time iTRAQ-based proteome profiling revealed the central metabolism involved in nitrogen starvation induced lipid accumulation in microalgae.Scientific reports · 2017Article
- Time-resolved Analysis of Proteome Dynamics by Tandem Mass Tags and Stable Isotope Labeling in Cell Culture (TMT-SILAC) Hyperplexing.Molecular & cellular proteomics : MCP · 2016Article
- Proteomic discovery of host kinase signaling in bacterial infections.Proteomics. Clinical applications · 2016Review
- Dissecting characteristics and dynamics of differentially expressed proteins during multistage carcinogenesis of human colorectal cancer.World journal of gastroenterology · 2016Article
- Proteomic profiling in MPTP monkey model for early Parkinson disease biomarker discovery.Biochimica et biophysica acta · 2015Article
- Quantitative proteomic analysis of histone modifications.Chemical reviews · 2015Review
- Evaluating multiplexed quantitative phosphopeptide analysis on a hybrid quadrupole mass filter/linear ion trap/orbitrap mass spectrometer.Analytical chemistry · 2015Article
- Review
- Comparing SILAC- and stable isotope dimethyl-labeling approaches for quantitative proteomics.Journal of proteome research · 2014Article
- MultiNotch MS3 enables accurate, sensitive, and multiplexed detection of differential expression across cancer cell line proteomes.Analytical chemistry · 2014Article
- Improved isobaric tandem mass tag quantification by ion mobility mass spectrometry.Rapid communications in mass spectrometry : RCM · 2014Article
- Miswiring the brain: Δ9-tetrahydrocannabinol disrupts cortical development by inducing an SCG10/stathmin-2 degradation pathway.The EMBO journal · 2014Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The accuracy of quantification obtained using the iTRAQ labelling methodology for measuring protein ratios more extreme than 1:1 was investigated. A comparison of nLC-ESI MSMS and nLC-MALDI MSMS analysis routes was performed. A fixed concentration of a standard six protein mix was spiked with two proteins at a range of concentrations. The two data analysis programmes, Mascot and ProteinPilot Paragon, were also compared. Whilst the lower ratios could be measured accurately, greater discrepancies were seen for the higher ratios, particularly by nLC-ESI MSMS. Filtering out the weaker reporter ion signals improved the accuracy of the ratios: this is likely due to several factors which are explored in more detail. Overall, analysis by nLC-MALDI MSMS followed by Mascot interpretation gave the most accurate results.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.