ArticleBiochimica et biophysica acta2010
Temperature-dependent phase behavior and protein partitioning in giant plasma membrane vesicles.
Article in Biochimica et biophysica acta, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
- Connexin 43-Enriched Vesicles Improve Synchronization in hiPSC-Derived Cardiomyocytes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- ER exit sites mediated by the COPII adaptor sec24D selectively recruit lipid raft-preferring proteins for rapid ER export.Nature communications · 2025Article
- Influence of the glycocalyx on the size and mechanical properties of plasma membrane-derived vesicles.Soft matter · 2025Article
- Lipid Peroxidation Drives Liquid-Liquid Phase Separation and Disrupts Raft Protein Partitioning in Biological Membranes.Journal of the American Chemical Society · 2024Article
- A Survey of Models of Cell Membranes: Toward a New Understanding of Membrane Organization.Cold Spring Harbor perspectives in biology · 2023Review
- Inhibition of neutral sphingomyelinase 2 impairs HIV-1 envelope formation and substantially delays or eliminates viral rebound.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Cholesterol- and actin-centered view of the plasma membrane: updating the Singer-Nicolson fluid mosaic model to commemorate its 50th anniversaryMolecular biology of the cell · 2023Article
- Micropatterning of functional lipid bilayer assays for quantitative bioanalysis.Biomicrofluidics · 2023Article
- Physical Concept to Explain the Regulation of Lipid Membrane Phase Separation under Isothermal Conditions.Life (Basel, Switzerland) · 2023Review
- High-Content Imaging Platform to Discover Chemical Modulators of Plasma Membrane Rafts.ACS central science · 2022Article
- Cholera Toxin as a Probe for Membrane Biology.Toxins · 2021Review
- Interleaflet Decoupling in a Lipid Bilayer at Excess Cholesterol Probed by Spectroscopic Ellipsometry and Simulations.The Journal of membrane biology · 2020Article
- Myelin-Associated MAL and PLP Are Unusual among Multipass Transmembrane Proteins in Preferring Ordered Membrane Domains.The journal of physical chemistry. B · 2020Article
- Structured clustering of the glycosphingolipid GM1 is required for membrane curvature induced by cholera toxin.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Peripheral myelin protein 22 preferentially partitions into ordered phase membrane domains.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Cell-Derived Plasma Membrane Vesicles Are Permeable to Hydrophilic Macromolecules.Biophysical journal · 2020Article
- Elastic deformations mediate interaction of the raft boundary with membrane inclusions leading to their effective lateral sorting.Scientific reports · 2020Article
- Chemically induced vesiculation as a platform for studying TMEM16F activity.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells.Journal of visualized experiments : JoVE · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Liquid-ordered (Lo) and liquid-disordered (Ld) phase coexistence has been suggested to partition the plasma membrane of biological cells into lateral compartments, allowing for enrichment or depletion of functionally relevant molecules. This dynamic partitioning might be involved in fine-tuning cellular signaling fidelity through coupling to the plasma membrane protein and lipid composition. In earlier work, giant plasma membrane vesicles, obtained by chemically induced blebbing from cultured cells, were observed to reversibly phase segregate at temperatures significantly below 37 degrees C. In this contribution, we compare the temperature dependence of fluid phase segregation in HeLa and rat basophilic leukemia (RBL) cells. We find an essentially monotonic temperature dependence of the number of phase-separated vesicles in both cell types. We also observe a strikingly broad distribution of phase transition temperatures in both cell types. The binding of peripheral proteins, such as cholera toxin subunit B (CTB), as well as Annexin V, is observed to modulate phase transition temperatures, indicating that peripheral protein binding may be a regulator for lateral heterogeneity in vivo. The partitioning of numerous signal protein anchors and full length proteins is investigated. We find Lo phase partitioning for several proteins assumed in the literature to be membrane raft associated, but observe deviations from this expectation for other proteins, including caveolin-1.
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