ArticleDevelopmental cell2010
Hsk1- and SCF(Pof3)-dependent proteolysis of S. pombe Ams2 ensures histone homeostasis and centromere function.
Article in Developmental cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 38 citations in OpenAlex.
- Identification and Validation of a Prognostic Model Based on Three MVI-Related Genes in Hepatocellular Carcinoma.International journal of biological sciences · 2022Article
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- Histone stress: an unexplored source of chromosomal instability in cancer?Current genetics · 2019Review
- Histone H2B Ubiquitylation Regulates Histone Gene Expression by Suppressing Antisense Transcription in Fission Yeast.Genetics · 2019Article
- A Dual Inhibitor of Cdc7/Cdk9 Potently Suppresses T Cell Activation.Frontiers in immunology · 2019Article
- Proteomics Analysis of SsNsd1-Mediated Compound Appressoria Formation inInternational journal of molecular sciences · 2018Article
- Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.Cell reports · 2018Article
- New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.PLoS genetics · 2018Article
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- Dependency of Heterochromatin Domains on Replication Factors.G3 (Bethesda, Md.) · 2018Article
- Genes Important forGenetics · 2018Article
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- Regulation of DNA replication-coupled histone gene expression.Oncotarget · 2017Review
- Characterisation of functional domains in fission yeast Ams2 that are required for core histone gene transcription.Scientific reports · 2016Article
- DDK dependent regulation of TOP2A at centromeres revealed by a chemical genetics approach.Nucleic acids research · 2016Article
- Mutation of histone H3 serine 86 disrupts GATA factor Ams2 expression and precise chromosome segregation in fission yeast.Scientific reports · 2015Article
- Review
- Cell cycle-regulated oscillator coordinates core histone gene transcription through histone acetylation.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Rad51-Rad52 mediated maintenance of centromeric chromatin in Candida albicans.PLoS genetics · 2014Article
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8 authors at 4 institutions in 2 countries.
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Abstract
Schizosaccharomyces pombe GATA factor Ams2 is responsible for cell cycle-dependent transcriptional activation of all the core histone genes peaking at G1/S phase. Intriguingly, its own protein level also fluctuates concurrently. Here, we show that Ams2 is ubiquitylated and degraded through the SCF (Skp1-Cdc53/Cullin-1-F-box) ubiquitin ligase, in which F box protein Pof3 binds this protein. Ams2 is phosphorylated at multiple sites, which is required for SCF(Pof3)-dependent proteolysis. Hsk1/Cdc7 kinase physically associates with and phosphorylates Ams2. Even mild overexpression of Ams2 induces constitutive histone expression and chromosome instability, and its toxicity is exaggerated when Hsk1 function is compromised. This is partly attributable to abnormal incorporation of canonical H3 into the central CENP-A/Cnp1-rich centromere, thereby reversing specific chromatin structures to apparently normal nucleosomes. We propose that Hsk1 plays a vital role during post S phase in genome stability via SCF(Pof3)-mediated degradation of Ams2, thereby maintaining centromere integrity.
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