ArticleChinese medicine2010
Generation and analysis of expressed sequence tags from a cDNA library of the fruiting body of Ganoderma lucidum.
Article in Chinese medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 17 citations in OpenAlex.
- Potential molecular mechanisms for fruiting body formation of Cordyceps illustrated in the case ofMycology · 2017Article
- Proteome exploration to provide a resource for the investigation of Ganoderma lucidum.PloS one · 2015Article
- Abundant and selective RNA-editing events in the medicinal mushroom Ganoderma lucidum.Genetics · 2014Article
- Generation and analysis of the expressed sequence tags from the mycelium of Ganoderma lucidum.PloS one · 2013Article
- Deep insight into the Ganoderma lucidum by comprehensive analysis of its transcriptome.PloS one · 2012Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLittle genomic or trancriptomic information on Ganoderma lucidum (Lingzhi) is known. This study aims to discover the transcripts involved in secondary metabolite biosynthesis and developmental regulation of G. lucidum using an expressed sequence tag (EST) library.
methodsA cDNA library was constructed from the G. lucidum fruiting body. Its high-quality ESTs were assembled into unique sequences with contigs and singletons. The unique sequences were annotated according to sequence similarities to genes or proteins available in public databases. The detection of simple sequence repeats (SSRs) was preformed by online analysis.
resultsA total of 1,023 clones were randomly selected from the G. lucidum library and sequenced, yielding 879 high-quality ESTs. These ESTs showed similarities to a diverse range of genes. The sequences encoding squalene epoxidase (SE) and farnesyl-diphosphate synthase (FPS) were identified in this EST collection. Several candidate genes, such as hydrophobin, MOB2, profilin and PHO84 were detected for the first time in G. lucidum. Thirteen (13) potential SSR-motif microsatellite loci were also identified.
conclusionThe present study demonstrates a successful application of EST analysis in the discovery of transcripts involved in the secondary metabolite biosynthesis and the developmental regulation of G. lucidum.
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