ArticleImmune network2010
Effects of IL-3 and SCF on Histamine Production Kinetics and Cell Phenotype in Rat Bone Marrow-derived Mast Cells.
Article in Immune network, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- IL-33 promotes gastric tumour growth in concert with activation and recruitment of inflammatory myeloid cells.Oncotarget · 2022Article
- Intercellular interactions between mast cells and stromal fibroblasts obtained from canine cutaneous mast cell tumours.Scientific reports · 2021Article
- Short-term in vitro culture of purity and highly functional rat bone marrow-derived mast cells.In vitro cellular & developmental biology. Animal · 2018Article
- Mast Cell, the Neglected Member of the Tumor Microenvironment: Role in Breast Cancer.Journal of immunology research · 2018Review
- Downregulation of iNOS, IL-1Journal of immunology research · 2018Article
- Biodegradable mesoporous calcium-magnesium silicate-polybutylene succinate scaffolds for osseous tissue engineering.International journal of nanomedicine · 2015Article
- Mast cells and basophils are essential for allergies: mechanisms of allergic inflammation and a proposed procedure for diagnosis.Acta pharmacologica Sinica · 2013Review
- Arthritis augments breast cancer metastasis: role of mast cells and SCF/c-Kit signaling.Breast cancer research : BCR · 2013Article
- In vitro modelling of rat mucosal mast cell function in Trichinella spiralis infection.Parasite immunology · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRat mast cells were regarded as a good model for mast cell function in immune response.
methodsRat bone marrow mast cells (BMMC) were prepared both by recombinant rat IL-3 (rrIL-3) and by recombinant mouse stem cell factor (rmSCF), and investigated for both proliferation and differentiation in time course. Rat BMMC was induced by culture of rat bone marrow cells (BMCs) in the presence of both rrIL-3 (5 ng/ml) and rmSCF (5 ng/ml). Culture media were changed 2 times per week with the cell number condition of 5x10(4)/ml in 6 well plate. Proliferation was analyzed by cell number and cell counting kit-8 (CCK-8) and differentiation was by rat mast cell protease (RMCP) II and histamine.
resultsCell proliferation rates reached a maximum at 8 or 11 days of culture and decreased thereafter. However, both RMCP II production and histamine synthesis peaked after 11 days of culture. By real time RT-PCR, the level of histidine decarboxylase mRNA was more than 500 times higher on culture day 11 than on culture day 5. By transmission electron microscopy, the cells were heterogeneous in size and contained cytoplasmic granules. Using gated flow cytometry, we showed that cultured BMCs expressed high levels of FcepsilonRI and the mast cell antigen, ganglioside, on culture day 11.
conclusionThese results indicate that rat BMMCs were generated by culturing BMCs in the presence of rrIL-3 and rmSCF and that the BMMCs have the characteristics of mucosal mast cells.
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