ArticleACS chemical neuroscience2010
Azi-isoflurane, a Photolabel Analog of the Commonly Used Inhaled General Anesthetic Isoflurane.
Article in ACS chemical neuroscience, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 39 citations in OpenAlex.
- The cryo-EM structure and physical basis for anesthetic inhibition of the THIK1 K2P channel.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Ketamine Metabolite (2ACS chemical neuroscience · 2021Article
- Article
- The Application of Fluorine-Containing Reagents in Structural Proteomics.Angewandte Chemie (International ed. in English) · 2020Review
- Volatile anesthetics isoflurane and sevoflurane directly target and attenuate Toll-like receptor 4 system.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Volatile anesthetics affect macrophage phagocytosis.PloS one · 2019Article
- Identification of binding sites contributing to volatile anesthetic effects on GABA type A receptors.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- X-Ray Crystallographic Studies for Revealing Binding Sites of General Anesthetics in Pentameric Ligand-Gated Ion Channels.Methods in enzymology · 2018Article
- Synthesis and pharmacological evaluation of neurosteroid photoaffinity ligands.European journal of medicinal chemistry · 2017Article
- Shedding Light on Anesthetic Mechanisms: Application of Photoaffinity Ligands.Anesthesia and analgesia · 2016Review
- Mechanisms revealed through general anesthetic photolabeling.Current anesthesiology reports · 2014Article
- Direct modulation of microtubule stability contributes to anthracene general anesthesia.Journal of the American Chemical Society · 2013Article
- NMR resolved multiple anesthetic binding sites in the TM domains of the α4β2 nAChR.Biochimica et biophysica acta · 2013Article
- In vivo activation of azipropofol prolongs anesthesia and reveals synaptic targets.The Journal of biological chemistry · 2013Article
- Volatile anesthetics, not intravenous anesthetic propofol bind to and attenuate the activation of platelet receptor integrin αIIbβ3.PloS one · 2013Article
- Novel activation of voltage-gated K(+) channels by sevoflurane.The Journal of biological chemistry · 2012Article
- Isoflurane binds and stabilizes a closed conformation of the leukocyte function-associated antigen-1.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Allyl m-trifluoromethyldiazirine mephobarbital: an unusually potent enantioselective and photoreactive barbiturate general anesthetic.Journal of medicinal chemistry · 2012Article
- General anesthetics predicted to block the GLIC pore with micromolar affinity.PLoS computational biology · 2012Article
- NMR structure and dynamics of a designed water-soluble transmembrane domain of nicotinic acetylcholine receptor.Biochimica et biophysica acta · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Volatility and low-affinity hamper an ability to define molecular targets of the inhaled anesthetics. Photolabels have proven to be a useful approach in this regard, although none have closely mimicked contemporary drugs. We report here the synthesis and validation of azi-isoflurane, a compound constructed by adding a diazirinyl moiety to the methyl carbon of the commonly used general anesthetic isoflurane. Azi-isoflurane is slightly more hydrophobic than isoflurane, and more potent in tadpoles. This novel compound inhibits Shaw2 K(+) channel currents similarly to isoflurane and binds to apoferritin with enhanced affinity. Finally, when irradiated at 300 nm, azi-isoflurane adducts to residues known to line isoflurane-binding sites in apoferritin and integrin LFA-1, the only proteins with isoflurane binding sites defined by crystallography. This reagent should allow rapid discovery of isoflurane molecular targets and binding sites within those targets.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.