ArticleThe Journal of biological chemistry2010
Mechanistic studies of hairpin to duplex conversion for trinucleotide repeat sequences.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Replication stress at microsatellites causes DNA double-strand breaks and break-induced replication.The Journal of biological chemistry · 2020Article
- Dynamic DNA Energy Landscapes and Substrate Complexity in Triplet Repeat Expansion and DNA Repair.Biomolecules · 2019Article
- Formation and processing of DNA damage substrates for the hNEIL enzymes.Free radical biology & medicine · 2017Review
- Unique Length-Dependent Biophysical Properties of Repetitive DNA.The journal of physical chemistry. B · 2016Article
- Impact of bulge loop size on DNA triplet repeat domains: Implications for DNA repair and expansion.Biopolymers · 2014Article
- Oligodeoxynucleotide binding to (CTG) · (CAG) microsatellite repeats inhibits replication fork stalling, hairpin formation, and genome instability.Molecular and cellular biology · 2013Article
- Instability of (CTG)n•(CAG)n trinucleotide repeats and DNA synthesis.Cell & bioscience · 2012Article
- Trinucleotide repeat DNA alters structure to minimize the thermodynamic impact of 8-oxo-7,8-dihydroguanine.Biochemistry · 2012Article
- Premutation huntingtin allele adopts a non-B conformation and contains a hot spot for DNA damage.Biochemical and biophysical research communications · 2011Article
- A small unstructured nucleic acid disrupts a trinucleotide repeat hairpin.Biochemical and biophysical research communications · 2011Article
- Structure of even/odd trinucleotide repeat sequences modulates persistence of non-B conformations and conversion to duplex.Biochemistry · 2011Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The expansion of a trinucleotide repeat sequence, such as CAG/CTG, has been pinpointed as the molecular basis for a number of neurodegenerative disorders. It has been proposed that as part of the expansion process, these repetitive sequences adopt non-B conformations such as hairpins. However, the prevalence of these hairpins and their contributions to the DNA expansion have not been well defined. In this work, we utilized a molecular beacon strategy to examine the stability of the (CAG)(10) hairpin and also its behavior in the presence of the complementary (CTG)(10) hairpin. We find that the two hairpins represent kinetically trapped species that can coexist but irreversibly convert to duplex upon thermal induction. Furthermore, as monitored by fluorescence and optical analysis, modifications to the base composition of either the loop or stem region have a profound effect on the ability of the trinucleotide repeat hairpins to convert to duplex. Additionally, the rate of duplex formation is also reduced with these loop and stem-modified hairpins. These results demonstrate that the trinucleotide repeat hairpins can convert to duplex via two independent mechanisms as follows: the loop-loop interactions found in kissing hairpins or the stem-stem interactions of a cruciform.
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