ArticleDifferentiation; research in biological diversity
Urothelium-derived Sonic hedgehog promotes mesenchymal proliferation and induces bladder smooth muscle differentiation.
Article in Differentiation; research in biological diversity. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Hedgehog pathway disruption alters urothelial differentiation and continence mechanisms in the female mouse lower urinary tract.American journal of physiology. Renal physiology · 2026Article
- Mechanisms and implications of epithelial cell plasticity in the bladder.Nature reviews. Urology · 2026Review
- Epithelial and mesenchymal compartments of the developing bladder and urethra display spatially distinct gene expression patterns.Developmental biology · 2025Article
- Interplay of SHH, WNT and BMP4 signaling regulates the development of the lamina propria in the murine ureter.Development (Cambridge, England) · 2025Article
- Transcription factor Tcf21 modulates urinary bladder size and differentiation.Development, growth & differentiation · 2024Article
- Review
- Heat-Inactivation of Fetal and Newborn Sera Did Not Impair the Expansion and Scaffold Engineering Potentials of Fibroblasts.Bioengineering (Basel, Switzerland) · 2021Article
- Spatiotemporal Expression of SHH/GLI Signaling in Human Fetal Bladder Development.Frontiers in pediatrics · 2021Article
- Roles for urothelium in normal and aberrant urinary tract development.Nature reviews. Urology · 2020Review
- Human Organ-Specific 3D Cancer Models Produced by the Stromal Self-Assembly Method of Tissue Engineering for the Study of Solid Tumors.BioMed research international · 2020Review
- Mesenchymal stromal cells modulate the molecular pattern of healing process in tissue-engineered urinary bladder: the microarray data.Stem cell research & therapy · 2019Article
- Fgfr2 is integral for bladder mesenchyme patterning and function.American journal of physiology. Renal physiology · 2017Article
- Stage- and subunit-specific functions of polycomb repressive complex 2 in bladder urothelial formation and regeneration.Development (Cambridge, England) · 2017Article
- An illustrated anatomical ontology of the developing mouse lower urogenital tract.Development (Cambridge, England) · 2015Article
- Fgfr2 is integral for bladder mesenchyme patterning and function.American journal of physiology. Renal physiology · 2015Article
- Analysis of the Sonic Hedgehog signaling pathway in normal and abnormal bladder development.PloS one · 2013Article
- Article
- Development of the human female reproductive tract.Differentiation; research in biological diversityReview
- Smooth muscle differentiation and patterning in the urinary bladder.Differentiation; research in biological diversityReview
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Induction of smooth muscle differentiation from bladder mesenchyme depends on signals that originate from the urothelium. We hypothesize Sonic hedgehog (Shh) is the urothelial signal that promotes bladder mesenchymal proliferation and induces bladder smooth muscle differentiation. Pregnant FVB mice were euthanized on embryonic day (E) 12.5 and fetal bladders were harvested. Two experimental protocols were utilized: Specimens were sized by serial sectioning. Cell counts were performed after trypsin digestion. Immunohistochemistry was performed to detect smooth muscle-specific protein expression. alpha-Actin expression was quantified using Western blot. All specimens were viable at 72h. BLM cultured without Shh survived but did not grow or undergo smooth muscle differentiation. IB cultured without Shh and BLM cultured with Shh grew and expressed smooth muscle proteins at 72h. IB cultured with Shh were larger and contained more cells than IB cultured without Shh (all p<0.05). Increasing Shh concentration from 48 to 480nM did not change bladder size, cell counts, or the level of alpha-actin expression. Prior to culture, IB did not express alpha-actin. After culture of IB in Shh-deficient media, alpha-actin was detected throughout the mesenchyme except in the submucosal layer. The IB submucosa was thinner after culture with 48nM Shh and smooth muscle completely obliterated the submucosa after culture with 480nM Shh. In fetal mouse bladders, urothelium-derived Shh is necessary for mesenchymal proliferation and smooth muscle differentiation. Shh concentration affects mesenchymal proliferation and patterning of bladder smooth muscle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.