ArticleCell host & microbe2010
Functional identification of the Plasmodium centromere and generation of a Plasmodium artificial chromosome.
Article in Cell host & microbe, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- A modular chromosomal passenger complex rewires chromosome segregation in Plasmodium berghei.Nature communications · 2026Article
- Precise gene regulation through transcriptional repression is essential for Plasmodium berghei asexual blood stage development.Nature communications · 2026Article
- Functional analyses of male-specific early-phosphorylated proteins reveal an intrinsically disordered protein required for microgamete release inFrontiers in cellular and infection microbiology · 2026Article
- Independent evolution of holocentric chromosomes in an early branching apicomplexan parasite.bioRxiv : the preprint server for biology · 2025Article
- Genetic manipulation for the non-model protozoaniScience · 2025Review
- Coordinated regulation of gene expression ineLife · 2024Article
- Transfection Models to InvestigatePathogens (Basel, Switzerland) · 2023Review
- Differentiation ofProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Genome-wide functional screening of drug-resistance genes in Plasmodium falciparum.Nature communications · 2022Article
- Composition and organization of kinetochores show plasticity in apicomplexan chromosome segregation.The Journal of cell biology · 2022Article
- Decrypting the complexity of the human malaria parasite biology through systems biology approaches.Frontiers in systems biology · 2022Article
- Activity of Plasmodium vivax promoter elements in Plasmodium knowlesi, and a centromere-containing plasmid that expresses NanoLuc throughout the parasite life cycle.Malaria journal · 2021Article
- The contribution of extrachromosomal DNA to genome plasticity in malaria parasites.Molecular microbiology · 2021Article
- Extrachromosomal DNA amplicons in antimalarial-resistant Plasmodium falciparum.Molecular microbiology · 2021Article
- Genetic Manipulation of Non-Falciparum Human Malaria Parasites.Frontiers in cellular and infection microbiology · 2021Review
- Real-time dynamics ofJournal of cell science · 2020Article
- Article
- A dual fluorescentCommunications biology · 2020Article
- Modeling Relapsing Malaria: Emerging Technologies to Study Parasite-Host Interactions in the Liver.Frontiers in cellular and infection microbiology · 2020Review
Corrections and comments
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- Erratum issued
Authors and funding
8 authors.
Funding
Abstract
The artificial chromosome represents a useful tool for gene transfer, both as cloning vectors and in chromosome biology research. To generate a Plasmodium artificial chromosome (PAC), we had to first functionally identify and characterize the parasite's centromere. A putative centromere (pbcen5) was cloned from chromosome 5 of the rodent parasite P. berghei based on a Plasmodium gene-synteny map. Plasmids containing pbcen5 were stably maintained in parasites during a blood-stage infection with high segregation efficiency, without drug pressure. pbcen5-containing plasmids were also stably maintained during parasite meiosis and mitosis in the mosquito. A linear PAC (L-PAC) was generated by integrating pbcen5 and telomere into a plasmid. The L-PAC segregated with a high efficiency and was stably maintained throughout the parasite's life cycle, as either one or two copies. These results suggest that L-PAC behaves like a Plasmodium chromosome, which can be exploited as an experimental research tool.
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