ArticleDiagnostic microbiology and infectious disease2010
Microfluidic platform versus conventional real-time polymerase chain reaction for the detection of Mycoplasma pneumoniae in respiratory specimens.
Article in Diagnostic microbiology and infectious disease, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Digital microfluidics comes of age: high-throughput screening to bedside diagnostic testing for genetic disorders in newborns.Expert review of molecular diagnostics · 2018Pooled it
- Nucleic acid amplification tests in digital microfluidics: the promise of next-generation point-of-care diagnostics.Microsystems & nanoengineering · 2025Review
- An All-in-One Platform for On-Site Multiplex Foodborne Pathogen Detection Based on Channel-Digital Hybrid Microfluidics.Biosensors · 2024Article
- A Digital Microfluidic RT-qPCR Platform for Multiple Detections of Respiratory Pathogens.Micromachines · 2022Article
- All-in-One Digital Microfluidics System for Molecular Diagnosis with Loop-Mediated Isothermal Amplification.Biosensors · 2022Article
- A Routing-Based Repair Method for Digital Microfluidic Biochips Based on an Improved Dijkstra and Improved Particle Swarm Optimization Algorithm.Micromachines · 2020Article
- Liquid Marble Actuator for Microfluidic Logic Systems.Scientific reports · 2018Article
- A digital microfluidic system for loop-mediated isothermal amplification and sequence specific pathogen detection.Scientific reports · 2017Article
- Digital Microfluidics for Nucleic Acid Amplification.Sensors (Basel, Switzerland) · 2017Review
- Comparison ofFrontiers in microbiology · 2016Article
- Etiological analysis and predictive diagnostic model building of community-acquired pneumonia in adult outpatients in Beijing, China.BMC infectious diseases · 2013Article
- Evaluation of a digital microfluidic real-time PCR platform to detect DNA of Candida albicans in blood.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Particles and microfluidics merged: perspectives of highly sensitive diagnostic detection.Mikrochimica acta · 2012Article
- Rapid molecular assays for specific detection and quantitation of Loa loa microfilaremia.PLoS neglected tropical diseases · 2011Article
- Elderly patients with community-acquired pneumonia: optimal treatment strategies.Drugs & aging · 2011Review
- Toward molecular parasitologic diagnosis: enhanced diagnostic sensitivity for filarial infections in mobile populations.Journal of clinical microbiology · 2011Article
- Effects of atypical infections with Mycoplasma and Chlamydia on asthma.Immunology and allergy clinics of North America · 2010Review
Corrections and comments
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Authors and funding
15 authors.
Funding
Abstract
Rapid, accurate diagnosis of community-acquired pneumonia (CAP) due to Mycoplasma pneumoniae is compromised by low sensitivity of culture and serology. Polymerase chain reaction (PCR) has emerged as a sensitive method to detect M. pneumoniae DNA in clinical specimens. However, conventional real-time PCR is not cost-effective for routine or outpatient implementation. Here, we evaluate a novel microfluidic real-time PCR platform (Advanced Liquid Logic, Research Triangle Park, NC) that is rapid, portable, and fully automated. We enrolled patients with CAP and extracted DNA from nasopharyngeal wash (NPW) specimens using a biotinylated capture probe and streptavidin-coupled magnetic beads. Each extract was tested for M. pneumoniae-specific DNA by real-time PCR on both conventional and microfluidic platforms using Taqman probe and primers. Three of 59 (5.0%) NPWs were positive, and agreement between the methods was 98%. The microfluidic platform was equally sensitive but 3 times faster and offers an inexpensive and convenient diagnostic test for microbial DNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.