Evidence map›Paper›PMID 20226363›Full record

ArticleJournal of bodywork and movement therapies2010

In vitro modeling of repetitive motion injury and myofascial release.

Kate R Meltzer, Thanh V Cao, Joseph F Schad, Hollis King, Scott T Stoll, Paul R Standley

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of bodywork and movement therapies, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed, 2 pooled it
2.9field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 2 syntheses or guidelines pooled it, 105 citations in OpenAlex.

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  3. Impact of 3-dimensional myofascial release on tumor necrosis factor (TNF) alpha biomarker in nonspecific low back pain: a randomised controlled trial.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 1 country.

Kate R MeltzerDepartment of Basic Medical Sciences, University of Arizona, College of Medicine, Phoenix, AZ 85004-2157, USA.
Thanh V Cao
Joseph F Schad
Hollis King
Scott T Stoll
Paul R Standley
University of Arizona · USA.T. Still University · USMidwestern University · USUniversity of North Texas · US

Funding

Project 3 - Pain-induced Sympathoexcitation: Effect of OMMU19AT002023 · NCCIH · UNIVERSITY OF NORTH TEXAS HLTH SCI CTR · PI SMITH, MICHAEL LAMAR · 2004 to 2009
$3.0M
NCCIH NIH HHS U19 AT002023
6 · The paper itself

Abstract

objectiveIn this study we modeled repetitive motion strain (RMS) and myofascial release (MFR) in vitro to investigate possible cellular and molecular mechanisms to potentially explain the immediate clinical outcomes associated with RMS and MFR.

methodCultured human fibroblasts were strained with 8h RMS, 60s MFR and combined treatment; RMS+MFR. Fibroblasts were immediately sampled upon cessation of strain and evaluated for cell morphology, cytokine secretions, proliferation, apoptosis, and potential changes to intracellular signaling molecules.

resultsRMS-induced fibroblast elongation of lameopodia, cellular decentralization, reduction of cell to cell contact and significant decreases in cell area to perimeter ratios compared to all other experimental groups (p<0.0001). Cellular proliferation indicated no change among any treatment group; however RMS resulted in a significant increase in apoptosis rate (p<0.05) along with increases in death-associated protein kinase (DAPK) and focal adhesion kinase (FAK) phosphorylation by 74% and 58% respectively, when compared to control. These responses were not observed in the MFR and RMS+MFR group. Of the 20 cytokines measured there was a significant increase in GRO secretion in the RMS+MFR group when compared to control and MFR alone.

conclusionOur modeled injury (RMS) appropriately displayed enhanced apoptosis activity and loss of intercellular integrity that is consistent with pro-apoptotic dapk-2 and FAK signaling. Treatment with MFR following RMS resulted in normalization in apoptotic rate and cell morphology both consistent with changes observed in dapk-2. These in vitro studies build upon the cellular evidence base needed to fully explain clinical efficacy of manual manipulative therapies.

Indexed as

FibroblastsAnalysis of VarianceApoptosisApoptosis Regulatory ProteinsCalcium-Calmodulin-Dependent Protein KinasesCase-Control StudiesCell ProliferationCells, CulturedCumulative Trauma DisordersCytokinesDeath-Associated Protein KinasesFocal Adhesion Kinase 1HumansMyofascial Pain SyndromesApoptosis Regulatory ProteinsCalcium-Calmodulin-Dependent Protein KinasesCytokinesDAPK2 protein, humanDeath-Associated Protein KinasesFocal Adhesion Kinase 1PTK2 protein, human

Identifiers

PMID20226363
PMCPMC2853774
OpenAlexW1987020227

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.