ArticleBMC microbiology2010
The PTS transporters of Lactobacillus gasseri ATCC 33323.
Article in BMC microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 52 citations in OpenAlex.
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- Lactobacilli and human dental caries: more than mechanical retention.Microbiology (Reading, England) · 2022Article
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- The Edible Plant Microbiome represents a diverse genetic reservoir with functional potential in the human host.Scientific reports · 2021Article
- Gentiobiose and cellobiose content in fresh and fermenting cucumbers and utilization of such disaccharides by lactic acid bacteria in fermented cucumber juice medium.Food science & nutrition · 2020Article
- Synbiotic Matchmaking in Lactobacillus plantarum: Substrate Screening and Gene-Trait Matching To Characterize Strain-Specific Carbohydrate Utilization.Applied and environmental microbiology · 2020Article
- Metabolically engineered Lactobacillus gasseri JCM 1131 as a novel producer of optically pure L- and D-lactate.World journal of microbiology & biotechnology · 2020Article
- Metabolomic and proteomic analysis of D-lactate-producing Lactobacillus delbrueckii under various fermentation conditions.Journal of industrial microbiology & biotechnology · 2018Article
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- Comparative Genomics of cpn60-Defined Enterococcus hirae Ecotypes and Relationship of Gene Content Differences to Competitive Fitness.Microbial ecology · 2016Article
- The Differential Proteome of the Probiotic Lactobacillus acidophilus NCFM Grown on the Potential Prebiotic Cellobiose Shows Upregulation of Two β -Glycoside Hydrolases.BioMed research international · 2015Article
- Article
- Distribution and functions of phosphotransferase system genes in the genome of the lactic acid bacterium Oenococcus oeni.Applied and environmental microbiology · 2013Article
- Metagenomic systems biology of the human gut microbiome reveals topological shifts associated with obesity and inflammatory bowel disease.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Transcriptional analysis of prebiotic uptake and catabolism by Lactobacillus acidophilus NCFM.PloS one · 2012Article
- A functional genomics approach to establish the complement of carbohydrate transporters in Streptococcus pneumoniae.PloS one · 2012Article
- Metabolic reconstruction for metagenomic data and its application to the human microbiome.PLoS computational biology · 2012Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLactobacilli can utilize a variety of carbohydrates which reflects the nutrient availability in their respective environments. A common lactobacilli in the human gastrointestinal tract, Lactobacillus gasseri, was selected for further study. The currently available annotation of the L. gasseri ATCC 33323 genome describes numerous putative genes involved in carbohydrate utilization, yet the specific functions of many of these genes remain unknown.
resultsAn enzyme I (EI) knockout strain revealed that a functional phosphotransferase transporter system (PTS) is required to ferment at least 15 carbohydrates. Analysis of the L. gasseri ATCC 33323 genome identified fifteen complete (containing all of the necessary subunits) PTS transporters. Transcript expression profiles in response to various carbohydrates (glucose, mannose, fructose, sucrose and cellobiose) were analyzed for the fifteen complete PTS transporters in L. gasseri. PTS 20 was induced 27 fold in the presence of sucrose and PTS 15 was induced 139 fold in the presence of cellobiose. No PTS transporter was induced by glucose, fructose or mannose. Insertional inactivation of PTS 15 and PTS 20 significantly impaired growth on cellobiose and sucrose, respectively. As predicted by bioinformatics, insertional inactivation of PTS 21 confirmed its role in mannose utilization.
conclusionsThe experiments revealed the extensive contribution of PTS transporters to carbohydrate utilization by L. gasseri ATCC 33323 and the general inadequacy of the annotated sugar specificity of lactobacilli PTS transporters.
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