ArticleMolecular & cellular proteomics : MCP2010
Quantitative proteomics analysis of Streptomyces coelicolor development demonstrates that onset of secondary metabolism coincides with hypha differentiation.
Article in Molecular & cellular proteomics : MCP, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Lon protease reprograms cellular physiology ofFrontiers in microbiology · 2026Article
- The Inhibition of Antibiotic Production inFrontiers in microbiology · 2020Article
- Quantitative Proteome and Phosphoproteome Analyses ofMolecular & cellular proteomics : MCP · 2018Article
- Dynamics of Pellet Fragmentation and Aggregation in Liquid-Grown Cultures ofFrontiers in microbiology · 2018Article
- Subcompartmentalization by cross-membranes during early growth of Streptomyces hyphae.Nature communications · 2016Article
- Proteomics analysis of global regulatory cascades involved in clavulanic acid production and morphological development in Streptomyces clavuligerus.Journal of industrial microbiology & biotechnology · 2016Article
- Detection of toxin proteins from Bacillus thuringiensis strain 4.0718 by strategy of 2D-LC-MS/MS.Current microbiology · 2015Article
- Differential proteomic profiling reveals regulatory proteins and novel links between primary metabolism and spinosad production in Saccharopolyspora spinosa.Microbial cell factories · 2014Article
- SdrA, a new DeoR family regulator involved in Streptomyces avermitilis morphological development and antibiotic production.Applied and environmental microbiology · 2013Article
- Pre-sporulation stages of Streptomyces differentiation: state-of-the-art and future perspectives.FEMS microbiology letters · 2013Review
- Saccharopolyspora erythraea's genome is organised in high-order transcriptional regions mediated by targeted degradation at the metabolic switch.BMC genomics · 2013Article
- Article
- Multiple-platform data integration method with application to combined analysis of microarray and proteomic data.BMC bioinformatics · 2012Article
- A probabilistic framework for peptide and protein quantification from data-dependent and data-independent LC-MS proteomics experiments.Omics : a journal of integrative biology · 2012Article
- Comparison of 4-plex to 8-plex iTRAQ quantitative measurements of proteins in human plasma samples.Journal of proteome research · 2012Article
- New insights on the development ofCurrent trends in microbiology · 2012Article
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5 authors.
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Abstract
Streptomyces species produce many clinically important secondary metabolites, including antibiotics and antitumorals. They have a complex developmental cycle, including programmed cell death phenomena, that makes this bacterium a multicellular prokaryotic model. There are two differentiated mycelial stages: an early compartmentalized vegetative mycelium (first mycelium) and a multinucleated reproductive mycelium (second mycelium) arising after programmed cell death processes. In the present study, we made a detailed proteomics analysis of the distinct developmental stages of solid confluent Streptomyces coelicolor cultures using iTRAQ (isobaric tags for relative and absolute quantitation) labeling and LC-MS/MS. A new experimental approach was developed to obtain homogeneous samples at each developmental stage (temporal protein analysis) and also to obtain membrane and cytosolic protein fractions (spatial protein analysis). A total of 345 proteins were quantified in two biological replicates. Comparative bioinformatics analyses revealed the switch from primary to secondary metabolism between the initial compartmentalized mycelium and the multinucleated hyphae.
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