ArticleJournal of pediatric surgery2010
Murine model of Hirschsprung-associated enterocolitis. I: phenotypic characterization with development of a histopathologic grading system.
Article in Journal of pediatric surgery, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 48 citations in OpenAlex.
- Molecular and Chromatin Accessibility Programs Underlying Epithelial Injury and Impaired Regeneration in Neonatal Necrotizing Enterocolitis.Cellular and molecular gastroenterology and hepatology · 2026Article
- Impairment of stromal-epithelial regenerative cross-talk in Hirschsprung disease primes for the progression to enterocolitis.Science translational medicine · 2025Article
- Mesenchymal Stem Cell Derived Exosomes Alleviates Hirschsprung-Associated Enterocolitis by Inhibiting AKT Phosphorylation in Macrophages Through miR-223.Stem cells international · 2025Article
- Acetylcholine from tuft cells promotes M2 macrophages polarization in Hirschsprung-associated enterocolitis.Frontiers in immunology · 2025Article
- Enteric neural stem cell transplant restores gut motility in mice with Hirschsprung disease.JCI insight · 2024Article
- Efficient enzyme-free method to assess the development and maturation of the innate and adaptive immune systems in the mouse colon.Scientific reports · 2024Article
- Paneth-like cells disruption and intestinal dysbiosis in the development of enterocolitis in an iatrogenic rectosigmoid hypoganglionosis rat model.Frontiers in surgery · 2024Article
- The mediating role of metabolites between gut microbiome and Hirschsprung disease: a bidirectional two-step Mendelian randomization study.Frontiers in pediatrics · 2024Article
- Comparation of robotic-assisted surgery and laparoscopic‑assisted surgery in children with Hirschsprung's disease: a single-centered retrospective study.BMC surgery · 2023Article
- Pathological and genetic characterization of foot and mouth disease viruses collected from cattle and water buffalo in Egypt.PloS one · 2023Article
- Current understanding of Hirschsprung-associated enterocolitis: Pathogenesis, diagnosis and treatment.Seminars in pediatric surgery · 2022Review
- Circulating exosomal microRNA-18a-5p accentuates intestinal inflammation in Hirschsprung-associated enterocolitis by targeting RORA.American journal of translational research · 2021Article
- Intestinal proinflammatory macrophages induce a phenotypic switch in interstitial cells of Cajal.The Journal of clinical investigation · 2020Article
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- Gut microbiota-mediated Gene-Environment interaction in the TashT mouse model of Hirschsprung disease.Scientific reports · 2019Article
- Gfra1 Underexpression Causes Hirschsprung's Disease and Associated Enterocolitis in Mice.Cellular and molecular gastroenterology and hepatology · 2019Article
- Bacterial Microbiome Dynamics in Post Pull-Through Hirschsprung-Associated Enterocolitis (HAEC): An Experimental Study Employing the Endothelin Receptor B-Null Mouse Model.Frontiers in surgery · 2018Article
- Guidelines for the diagnosis and management of Hirschsprung-associated enterocolitis.Pediatric surgery international · 2017Review
- Established and emerging concepts in Hirschsprung's-associated enterocolitis.Pediatric surgery international · 2016Review
- Enterocolitis causes profound lymphoid depletion in endothelin receptor B- and endothelin 3-null mouse models of Hirschsprung-associated enterocolitis.European journal of immunology · 2015Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
purposeThe aim of the study was to characterize enterocolitis in the Ednrb-null (Ednrb-/-) mouse with aganglionosis of the colon and to develop and validate a semiquantitative histopathologic grading system to assess enterocolitis.
methodsWe isolated colon and ileal specimens of Ednrb-/- and control mice (Ednrb+/+) and performed histochemical staining (H&E) on tissue sections. After establishing inflammation grading criteria, 2 blinded pathologists independently assessed the severity and depth of inflammation of proximal colon segments on 2 separate occasions. Interclass correlations (ICCs) and coefficient of variation (CV) were calculated to determine interrater and intrarater agreement. We then prospectively applied the enterocolitis grading system to Ednrb-/- mice that became clinically ill. A cohort of Ednrb-/- mice were observed until they developed clinical illness, at which time they were euthanized and had multiple organ homogenates cultured for bacteria, and colon and small bowel were histopathologically graded for enterocolitis. Spearman's rank correlations comparing enterocolitis scores with level of bacteremia were performed.
resultsIntra- and interrater ICCs of the histologic scoring system were satisfactory (0.61 and 0.94, respectively), as were intra- and interrater CVs (18% and 9%, respectively). Of the Ednrb-/- mice, 65% developed bacteremia. Those with bacteremia had significantly higher enterocolitis scores than those without bacteremia (P < .01). Ednrb-/- mice that developed bacteremia showed a strong positive correlation between total enterocolitis scores and number of bacterial colony forming units in peritoneal lavage, liver, kidney, and aerobic spleen.
conclusionsThe Ednrb-/- mouse with aganglionosis develops enterocolitis and has features similar to Hirschsprung-associated enterocolitis in humans. Our grading system is a reliable way to assess enterocolitis. By performing microsurgical pull-through, we can now perform controlled, hypothesis-driven, mechanistic studies to evaluate etiologic factors affecting enterocolitis in the Ednrb-/- mouse.
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